MicroRNA: function, detection, and bioanalysis

H Dong, J Lei, L Ding, Y Wen, H Ju, X Zhang - Chemical reviews, 2013‏ - ACS Publications
Mature microRNAs (miRNAs) are a class of evolutionally conserved, single-stranded, small
(approximately 19− 23 nucleotides), endogenously expressed, and non-protein-coding …

MicroRNA profiling: approaches and considerations

CC Pritchard, HH Cheng, M Tewari - Nature Reviews Genetics, 2012‏ - nature.com
MicroRNAs (miRNAs) are small RNAs that post-transcriptionally regulate the expression of
thousands of genes in a broad range of organisms in both normal physiological contexts …

microRNA biosensors: Opportunities and challenges among conventional and commercially available techniques

T Kilic, A Erdem, M Ozsoz, S Carrara - Biosensors and Bioelectronics, 2018‏ - Elsevier
As being the most extensively studied, non-coding, evolutionary conserved, post-
transcriptional gene regulators of genome, microRNAs (miRNAs) have taken great attention …

Micro total analysis systems for cell biology and biochemical assays

ML Kovarik, PC Gach, DM Ornoff, Y Wang… - Analytical …, 2012‏ - ACS Publications
Novel applications of micro total analysis systems (μTAS) are addressing fundamental
biological questions, creating new biomedical reagents, and develo** innovative cell and …

Purification of nucleic acids using isotachophoresis

A Rogacs, LA Marshall, JG Santiago - Journal of Chromatography A, 2014‏ - Elsevier
Reviewed are methods of nucleic acid (NA) extraction and sample preparation using an
electrophoretic purification and focusing method called isotachophoresis (ITP). ITP requires …

Microfluidic isotachophoresis: A review

P Smejkal, D Bottenus, MC Breadmore… - …, 2013‏ - Wiley Online Library
Electromigration methods including CE and ITP are attractive for incorporation in microfluidic
devices because they are relatively easily adaptable to miniaturization. After its popularity in …

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2012–2014)

MC Breadmore, RM Tubaon, AI Shallan… - …, 2015‏ - Wiley Online Library
One of the most cited limitations of capillary (and microchip) electrophoresis is the poor
sensitivity. This review continues to update this series of biannual reviews, first published in …

MicroRNA profiling by simultaneous selective isotachophoresis and hybridization with molecular beacons

A Persat, JG Santiago - Analytical chemistry, 2011‏ - ACS Publications
We present and demonstrate a novel assay for the detection and quantification of microRNA
(miRNA) that leverages isotachophoresis (ITP) and molecular beacon (MB) hybridization …

On-chip separation and analysis of RNA and DNA from single cells

H Shintaku, H Nishikii, LA Marshall, H Kotera… - Analytical …, 2014‏ - ACS Publications
The simultaneous analysis of RNA and DNA of single cells remains a challenge as these
species have very similar physical and biochemical properties and can cross-contaminate …

Integrated printed circuit board device for cell lysis and nucleic acid extraction

LA Marshall, LL Wu, S Babikian, M Bachman… - Analytical …, 2012‏ - ACS Publications
Preparation of raw, untreated biological samples remains a major challenge in microfluidics.
We present a novel microfluidic device based on the integration of printed circuit boards and …