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Top-down proteomics
Proteoforms, which arise from post-translational modifications, genetic polymorphisms and
RNA splice variants, play a pivotal role as drivers in biology. Understanding proteoforms is …
RNA splice variants, play a pivotal role as drivers in biology. Understanding proteoforms is …
Top-down proteomics and the challenges of true proteoform characterization
Top-down proteomics (TDP) aims to identify and profile intact protein forms (proteoforms)
extracted from biological samples. True proteoform characterization requires that both the …
extracted from biological samples. True proteoform characterization requires that both the …
Influence of different sample preparation approaches on proteoform identification by top-down proteomics
Top-down proteomics using mass spectrometry facilitates the identification of intact
proteoforms, that is, all molecular forms of proteins. Multiple past advances have lead to the …
proteoforms, that is, all molecular forms of proteins. Multiple past advances have lead to the …
Mass spectrometry-intensive top-down proteomics: an update on technology advancements and biomedical applications
Proteoforms are all forms of protein molecules from the same gene because of variations at
the DNA, RNA, and protein levels, eg, alternative splicing and post-translational …
the DNA, RNA, and protein levels, eg, alternative splicing and post-translational …
PEPPI-MS: gel-based sample pre-fractionation for deep top-down and middle-down proteomics
Top-down analysis of intact proteins and middle-down analysis of proteins subjected to
limited digestion require efficient detection of traces of proteoforms in samples, necessitating …
limited digestion require efficient detection of traces of proteoforms in samples, necessitating …
Proteomics—the state of the field: the definition and analysis of proteomes should Be based in reality, not convenience
With growing recognition and acknowledgement of the genuine complexity of proteomes, we
are finally entering the post-proteogenomic era. Routine assessment of proteomes as …
are finally entering the post-proteogenomic era. Routine assessment of proteomes as …
[HTML][HTML] Present and future perspectives on mass spectrometry for clinical microbiology
In the last decade, MALDI-TOF Mass Spectrometry (MALDI-TOF MS) has been introduced
and broadly accepted by clinical laboratory laboratories throughout the world as a powerful …
and broadly accepted by clinical laboratory laboratories throughout the world as a powerful …
High-throughput deconvolution of native protein mass spectrometry imaging data sets for mass domain analysis
Protein mass spectrometry imaging (MSI) with electrospray-based ambient ionization
techniques, such as nanospray desorption electrospray ionization (nano-DESI), generates …
techniques, such as nanospray desorption electrospray ionization (nano-DESI), generates …
Immunoaffinity Intact Top-Down Mass Spectrometry for Quantification of Neuron-Specific Enolase Gamma, a Low-Abundance Protein Biomarker
SAH van den Wildenberg, SAAM Genet… - Analytical …, 2024 - ACS Publications
Quantification of intact proteins in serum by liquid chromatography high-resolution mass
spectrometry (HRMS) may be a useful alternative to bottom-up LC-MS or conventional …
spectrometry (HRMS) may be a useful alternative to bottom-up LC-MS or conventional …
FREE: Enhanced Feature Representation for Isotopic Envelope Evaluation in Top-Down Mass Spectra Deconvolution
The aim of deconvolution of top-down mass spectra is to recognize monoisotopic peaks from
the experimental envelopes in raw mass spectra. So accurate assessment of similarity …
the experimental envelopes in raw mass spectra. So accurate assessment of similarity …