Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts
In this review, we detail the efforts performed to couple the purification and the
immobilization of industrial enzymes in a single step. The use of antibodies, the …
immobilization of industrial enzymes in a single step. The use of antibodies, the …
[HTML][HTML] Orientation and characterization of immobilized antibodies for improved immunoassays
Orientation of surface immobilized capture proteins, such as antibodies, plays a critical role
in the performance of immunoassays. The sensitivity of immunodiagnostic procedures is …
in the performance of immunoassays. The sensitivity of immunodiagnostic procedures is …
Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance
K Hernandez, R Fernandez-Lafuente - Enzyme and microbial technology, 2011 - Elsevier
Mutagenesis and immobilization are usually considered to be unrelated techniques with
potential applications to improve protein properties. However, there are several reports …
potential applications to improve protein properties. However, there are several reports …
Site-directed antibody immobilization techniques for immunosensors
A Makaraviciute, A Ramanaviciene - Biosensors and Bioelectronics, 2013 - Elsevier
Immunosensor sensitivity, regenerability, and stability directly depend on the type of
antibodies used for the immunosensor design, quantity of immobilized molecules, remaining …
antibodies used for the immunosensor design, quantity of immobilized molecules, remaining …
Microbial tyrosinases: promising enzymes for pharmaceutical, food bioprocessing, and environmental industry
Tyrosinase is a natural enzyme and is often purified to only a low degree and it is involved in
a variety of functions which mainly catalyse the o‐hydroxylation of monophenols into their …
a variety of functions which mainly catalyse the o‐hydroxylation of monophenols into their …
Significance of antibody orientation unraveled: well-oriented antibodies recorded high binding affinity
N Tajima, M Takai, K Ishihara - Analytical chemistry, 2011 - ACS Publications
To investigate the effect of antibody orientation on its immunological activities, we developed
a novel and versatile platform consisting of a well-defined phospholipid polymer surface on …
a novel and versatile platform consisting of a well-defined phospholipid polymer surface on …
Bacterial tyrosinases and their applications
Tyrosinases with different physico-chemical properties have been identified from various
bacterial phyla such as Actinobacteria and Proteobacteria and their production is often …
bacterial phyla such as Actinobacteria and Proteobacteria and their production is often …
Biofabrication to build the biology–device interface
The last century witnessed spectacular advances in both microelectronics and
biotechnology yet there was little synergy between the two. A challenge to their integration is …
biotechnology yet there was little synergy between the two. A challenge to their integration is …
From protein features to sensing surfaces
G Faccio - Sensors, 2018 - mdpi.com
Proteins play a major role in biosensors in which they provide catalytic activity and specificity
in molecular recognition. However, the immobilization process is far from straightforward as …
in molecular recognition. However, the immobilization process is far from straightforward as …
Tyrosinase-mediated grafting and crosslinking of natural phenols confers functional properties to chitosan
Physics and chemistry underpinned the remarkable advances in materials science that
occurred during the 20th century, while biology is poised to provide the scientific …
occurred during the 20th century, while biology is poised to provide the scientific …