The emergence and spread of antibiotic resistance is one of the biggest challenges in public
health. There is an urgent need to discover novel agents against the occurrence of multidrug …

Over the last decade, single-molecule detection has been successfully utilized in the life
sciences and materials science. Yet, single-molecule measurements only yield meaningful …

We report a new approach to selectively deliver antimicrobials to the sites of bacterial
infections by utilizing bacterial toxins to activate drug release from gold nanoparticle …

Proteins attain their function only after folding into a highly organized three-dimensional
structure. Much remains to be learned about the mechanisms of folding of large multidomain …

Single-molecule fluorescence techniques have emerged as powerful tools to study
biological processes at the molecular level. This review describes the application of these …

Single-molecule fluorescence spectroscopy reveals the real time dynamics that occur during
biomolecular interactions that would otherwise be hidden by the ensemble average. It also …

Fluorescence imaging is used to study the dynamics of a wide variety of single molecules in
solution or attached to a surface. Two key challenges in this pursuit are (1) to image …

The in vitro studies of membrane fusion mediated by soluble N-ethylmaleimide-sensitive
factor attachment protein receptors (SNAREs) have primarily been conducted by following …

We developed a system to reversibly encapsulate small numbers of molecules in an array of
nanofabricated “dimples”. This system enables highly parallel, long-term, and attachment …

In this article, we summarize the knowledge and best practices learned from bulk and single-
molecule measurements to address some of the frequently experienced difficulties in single …