Although there is now an extensive understanding of the diversity of microbial life on earth
through culture-independent metagenomic DNA sequence analyses, the isolation and …

Raman microspectroscopy offers microbiologists a rapid and non-destructive technique to
assess the chemical composition of individual live microorganisms in near real time. In this …

Antimicrobial resistance (AMR) has become a global clinical problem in recent years. With
the discovery of antibiotics, infections were not a deadly problem for clinicians as they used …

The function of cells in their native habitat often cannot be reliably predicted from genomic
data or from physiology studies of isolates. Traditional experimental approaches to study the …

Viability and metabolic assays are commonly used as proxies to assess the overall
metabolism of microorganisms. The variety of these assays combined with little information …

Bacterial detection is a highly topical research area, because various fields of application
will benefit from the progress being made. Consequently, new and innovative strategies …

Optical microscopy techniques have emerged as a cornerstone of biomedical research,
capable of probing the cellular functions of a vast range of substrates, whilst being minimally …

Highlights•Single cell Raman spectra (SCRS) are label-free and intrinsic biochemical
profiles of single microbial cells.•A number of bands of SCRS shift if cells are labeled with …

The inherent heterogeneity in cell populations has become of great interest and importance
as analytical techniques have improved over the past decades. With the advent of …

To combat the spread of antibiotic resistance, methods that quantitatively assess the
metabolism-inhibiting effects of drugs in a rapid and culture-independent manner are …