Approaches to DNA mutagenesis: an overview
MM Ling, BH Robinson - Analytical biochemistry, 1997 - Elsevier
Mutagenesis of proteins is of paramount importance chain reaction (PCR) or non-PCR, has
revolutionized the means by which mutants are obtained (2, 3). Mutafor understanding the …
revolutionized the means by which mutants are obtained (2, 3). Mutafor understanding the …
Engineering biosynthetic enzymes for industrial natural product synthesis
Covering: 2000 to 2020 Natural products and their derivatives are commercially important
medicines, agrochemicals, flavors, fragrances, and food ingredients. Industrial strategies to …
medicines, agrochemicals, flavors, fragrances, and food ingredients. Industrial strategies to …
Randomization of genes by PCR mutagenesis.
RC Cadwell, GF Joyce - Genome research, 1992 - genome.cshlp.org
A modified polymerase chain reaction (PCR) was developed to introduce random point
mutations into cloned genes. The modifications were made to decrease the fidelity of Taq …
mutations into cloned genes. The modifications were made to decrease the fidelity of Taq …
Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers
Background RNA-seq and small RNA-seq are powerful, quantitative tools to study gene
regulation and function. Common high-throughput sequencing methods rely on polymerase …
regulation and function. Common high-throughput sequencing methods rely on polymerase …
Directed evolution of an RNA enzyme
AA Beaudry, GF Joyce - Science, 1992 - science.org
An in vitro evolution procedure was used to obtain RNA enzymes with a particular catalytic
function. A population of 1013 variants of the Tetrahymena ribozyme, a group I ribozyme that …
function. A population of 1013 variants of the Tetrahymena ribozyme, a group I ribozyme that …
Multiple Ras functions can contribute to mammalian cell transformation
MA White, C Nicolette, A Minden, A Polverino… - Cell, 1995 - cell.com
We have developed a generalized approach, using two hybrid interactions, to isolate Ha-
Ras effector loop mutations that separate the ability of Ha-Ras to interact with different …
Ras effector loop mutations that separate the ability of Ha-Ras to interact with different …
The Saccharomyces telomere-binding protein Cdc13p interacts with both the catalytic subunit of DNA polymerase α and the telomerase-associated Est1 protein
H Qi, VA Zakian - Genes & development, 2000 - genesdev.cshlp.org
Saccharomyces telomeres consist of∼ 350 bp of C1-3A/TG1-3 DNA. Most of this∼ 350 bp is
replicated by standard, semiconservative DNA replication. After conventional replication, the …
replicated by standard, semiconservative DNA replication. After conventional replication, the …
Precise prediction of promoter strength based on a de novo synthetic promoter library coupled with machine learning
Promoters are one of the most critical regulatory elements controlling metabolic pathways.
However, the fast and accurate prediction of promoter strength remains challenging, leading …
However, the fast and accurate prediction of promoter strength remains challenging, leading …
Direct random mutagenesis of gene-sized DNA fragments using polymerase chain reaction
M Fromant, S Blanquet, P Plateau - Analytical biochemistry, 1995 - Elsevier
The polymerase chain reaction (PCR) can be used to amplify a DNA fragment with the
concomitant creation of numerous mutations provided that one dNTP substrate is in excess …
concomitant creation of numerous mutations provided that one dNTP substrate is in excess …
Glucose regulates protein interactions within the yeast SNF1 protein kinase complex.
R Jiang, M Carlson - Genes & development, 1996 - genesdev.cshlp.org
The SNF1 protein kinase is broadly conserved in eukaryotes and has been implicated in
responses to environmental and nutritional stress. In yeast, the SNF1 kinase has a central …
responses to environmental and nutritional stress. In yeast, the SNF1 kinase has a central …