[HTML][HTML] Methods of inactivation of highly pathogenic viruses for molecular, serology or vaccine development purposes

S Elveborg, VM Monteil, A Mirazimi - Pathogens, 2022‏ - mdpi.com
The handling of highly pathogenic viruses, whether for diagnostic or research purposes,
often requires an inactivation step. This article reviews available inactivation techniques …

Inactivated virus vaccines from chemistry to prophylaxis: merits, risks and challenges

I Delrue, D Verzele, A Madder… - Expert review of …, 2012‏ - Taylor & Francis
The aim of this review is to make researchers aware of the benefits of an efficient quality
control system for prediction of a developed vaccine's efficacy. Two major goals should be …

HIV-1 Env trimers asymmetrically engage CD4 receptors in membranes

W Li, Z Qin, E Nand, MW Grunst, JR Grover, JW Bess Jr… - Nature, 2023‏ - nature.com
Abstract Human immunodeficiency virus 1 (HIV-1) infection is initiated by binding of the viral
envelope glycoprotein (Env) to the cell-surface receptor CD4,,–. Although high-resolution …

N6-methyladenosine of HIV-1 RNA regulates viral infection and HIV-1 Gag protein expression

N Tirumuru, BS Zhao, W Lu, Z Lu, C He, L Wu - elife, 2016‏ - elifesciences.org
The internal N 6-methyladenosine (m6A) methylation of eukaryotic nuclear RNA controls
post-transcriptional gene expression, which is regulated by methyltransferases (writers) …

Distribution and three-dimensional structure of AIDS virus envelope spikes

P Zhu, J Liu, J Bess Jr, E Chertova, JD Lifson, H Grisé… - Nature, 2006‏ - nature.com
Envelope glycoprotein (Env) spikes on AIDS retroviruses initiate infection of host cells and
are therefore targets for vaccine development. Though crystal structures for partial Env …

Molecular architecture of native HIV-1 gp120 trimers

J Liu, A Bartesaghi, MJ Borgnia, G Sapiro… - Nature, 2008‏ - nature.com
The envelope glycoproteins (Env) of human and simian immunodeficiency viruses (HIV and
SIV, respectively) mediate virus binding to the cell surface receptor CD4 on target cells to …

Human Immunodeficiency Virus Type 1 env Clones from Acute and Early Subtype B Infections for Standardized Assessments of Vaccine-Elicited Neutralizing …

M Li, F Gao, JR Mascola, L Stamatatos… - Journal of …, 2005‏ - journals.asm.org
Induction of broadly cross-reactive neutralizing antibodies is a high priority for AIDS vaccine
development but one that has proven difficult to be achieved. While most immunogens …

[HTML][HTML] Cryo-ET of Env on intact HIV virions reveals structural variation and positioning on the Gag lattice

VM Prasad, DP Leaman, KN Lovendahl, JT Croft… - Cell, 2022‏ - cell.com
HIV-1 Env mediates viral entry into host cells and is the sole target for neutralizing
antibodies. However, Env structure and organization in its native virion context has eluded …

Effector memory T cell responses are associated with protection of rhesus monkeys from mucosal simian immunodeficiency virus challenge

SG Hansen, C Vieville, N Whizin, L Coyne-Johnson… - Nature medicine, 2009‏ - nature.com
The rapid onset of massive, systemic viral replication during primary HIV or simian
immunodeficiency virus (SIV) infection and the immune evasion capabilities of these viruses …

Electron tomography analysis of envelope glycoprotein trimers on HIV and simian immunodeficiency virus virions

P Zhu, E Chertova, J Bess Jr, JD Lifson… - Proceedings of the …, 2003‏ - pnas.org
We used electron tomography to directly visualize trilobed presumptive envelope (env)
glycoprotein structures on the surface of negatively stained HIV type 1 (HIV-1) and simian …