Non-immunoglobulin (non-Ig) scaffolds are, in contrast to antibodies, small single-domain
proteins that require no post-translational modification, often lack disulfide bonds, and can …

High‐resolution experimental structural determination of protein–protein interactions has led
to valuable mechanistic insights, yet due to the massive number of interactions and …

Adaptive immunity in jawless fishes is based on antigen recognition by three types of
variable lymphocyte receptors (VLRs) composed of variable leucine-rich repeats, which are …

A central question in protein evolution is the extent to which naturally occurring proteins
sample the space of folded structures accessible to the polypeptide chain. Repeat proteins …

The success of protein engineering and design has extensively expanded the protein space,
which presents a promising strategy for creating next-generation proteins of diverse …

We have designed a novel non-antibody scaffold protein, termed Adhiron, based on a
phytocystatin consensus sequence. The Adhiron scaffold shows high thermal stability (T m …

The demand for recombinant therapeutic antibodies and Fc-fusion proteins is expected to
increase in the years to come. Hence, extensive efforts are concentrated on improving the …

Recreating the most critical aspects of the native extracellular matrix (ECM) is fundamental
to understand and control the processes regulating cell fate and cell function. From the ill …

Highlights•Scaffold libraries have now combined concave and convex paratopes.•Scaffold
proteins have shown new uses from sensors to viral retargeting.•Bispecific IgG molecules …

The use of traditional tools for the targeted delivery of nanostructures, such as antibodies,
transferrin, lectins, or aptamers, often leads to an entire range of undesirable effects. The …