The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …

Microalgae are microorganisms capable of producing bioactive compounds using
photosynthesis. Microalgae contain a variety of high value-added natural pigments such as …

Promoter editing represents an innovative approach to introduce quantitative trait variation
(QTV) in crops. However, an efficient promoter editing system for QTV needs to be …

CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided
genome engineering in plants. However, these systems are mostly used separately, leaving …

Background CRISPR/Cas-derived base editor enables precise editing of target sites and
has been widely used for basic research and crop genetic improvement. However, the …

RNA-guided CRISPR activation (CRISPRa) systems have been developed in plants.
However, the simultaneous activation of multiple genes remains challenging. Here, we …

Climate change is a threat to global food security due to the reduction of crop productivity
around the globe. Food security is a matter of concern for stakeholders and policymakers as …

CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …

Precise replacement of an allele with an elite allele controlling an important agronomic trait
in a predefined manner by gene editing technologies is highly desirable in crop …

The CRISPR‐Cas‐based genome editing field in plants is expanding rapidly. Editing plant
promoters to obtain cis‐regulatory alleles with altered expression levels or patterns of target …