Metagenomics: novel enzymes from non-culturable microbes

F Berini, C Casciello, GL Marcone… - FEMS microbiology …, 2017 - academic.oup.com
In the transition to the post-petroleum economy, there is a growing demand for novel
enzymes with high process performances to replace traditional chemistry with a more …

Role of metagenomics in prospecting novel endoglucanases, accentuating functional metagenomics approach in second-generation biofuel production: a review

NPP Pabbathi, A Velidandi, T Tavarna, S Gupta… - Biomass Conversion …, 2021 - Springer
As the fossil fuel reserves are depleting rapidly, there is a need for alternate fuels to meet the
day to day mounting energy demands. As fossil fuel started depleting, a quest for alternate …

A novel high glucose-tolerant β-glucosidase: targeted computational approach for metagenomic screening

S Ariaeenejad, S Nooshi-Nedamani… - … in bioengineering and …, 2020 - frontiersin.org
The rate-limiting component of cellulase for efficient degradation of lignocellulosic biomass
through the enzymatic route depends on glucosidase's sensitivity to the end product …

Hydroxycinnamic acid extraction from multiple lignocellulosic sources: correlations with substrate composition and taxonomy for flavoring and antioxidant applications

R Tramontina, E Scopel, VGK Cardoso… - Journal of Agricultural …, 2024 - ACS Publications
The extraction of hydroxycinnamic acids (HCADs) is a strategy for lignocellulosic biomass
valorization due to their high value-added nature and the possibility of application as …

Metagenomic approaches as a tool to unravel promising biocatalysts from natural resources: soil and water

J Sousa, SC Silvério, AMA Costa, LR Rodrigues - Catalysts, 2022 - mdpi.com
Natural resources are considered a promising source of microorganisms responsible for
producing biocatalysts with great relevance in several industrial areas. However, a …

Biochemical characterization of a novel monospecific endo-β-1, 4-glucanase belonging to GH family 5 from a rhizosphere metagenomic library

A Wierzbicka-Woś, R Henneberger… - Frontiers in …, 2019 - frontiersin.org
Cellulases have a broad range of different industrial applications, ranging from food and
beverages to pulp and paper and the biofuels area. Here a metagenomics based strategy …

[HTML][HTML] In silico characterization of the GH5-cellulase family from uncultured microorganisms: physicochemical and structural studies

RE Sanjaya, KDA Putri, A Kurniati, A Rohman… - Journal of Genetic …, 2021 - Elsevier
Background Hydrolysis of cellulose-based biomass by cellulases produce fermented sugar
for making biofuels, such as bioethanol. Cellulases hydrolyze the β-1, 4-glycosidic linkage of …

Metagenomics in the search for industrial enzymes

T Ahmad, RS Singh, G Gupta, A Sharma… - Advances in Enzyme …, 2019 - Elsevier
From bacteria to man, all living organisms are built and maintained by biocatalysts called
enzymes. Microorganisms are preferential sources for the production of industrial enzymes …

Recombinant thermo-alkali-stable endoglucanase of Myceliopthora thermophila BJA (rMt-egl): biochemical characteristics and applicability in enzymatic …

P Phadtare, S Joshi, T Satyanarayana - International Journal of Biological …, 2017 - Elsevier
Codon adaptation index (CAI) of a 1263 bp long endoglucanase encoding gene from the
thermophilic mould Myceliopthora thermophile BJA has been improved from 0.44 to 0.76 by …

Current status and potential applications of underexplored prokaryotes

KM Goh, S Shahar, KG Chan, CS Chong, SI Amran… - Microorganisms, 2019 - mdpi.com
Thousands of prokaryotic genera have been published, but methodological bias in the study
of prokaryotes is noted. Prokaryotes that are relatively easy to isolate have been well …