Recent progress of SELEX methods for screening nucleic acid aptamers

C Zhu, Z Feng, H Qin, L Chen, M Yan, L Li, F Qu - Talanta, 2024 - Elsevier
Nucleic acid aptamers are oligonucleotide sequences screened by an in vitro methodology
called Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Known as …

Aptamer-based biosensors for virus protein detection

B Lou, Y Liu, M Shi, J Chen, K Li, Y Tan, L Chen… - TrAC Trends in …, 2022 - Elsevier
Virus threatens life health seriously. The accurate early diagnosis of the virus is vital for
clinical control and treatment of virus infection. Aptamers are small single-stranded …

Development of Nucleic‐Acid‐Based Electrochemical Biosensors for Clinical Applications

Z Zhang, P Sen, BR Adhikari, Y Li… - Angewandte …, 2022 - Wiley Online Library
Nucleic acids are remarkable molecules. In addition to Watson–Crick base pairing, the
different structural motifs of these molecules can bind non‐nucleic acid targets or catalyze …

Translation of aptamers toward clinical diagnosis and commercialization

S Liu, Y Xu, X Jiang, H Tan, B Ying - Biosensors and Bioelectronics, 2022 - Elsevier
The dominance of antibodies in diagnostics has gradually changed following the discovery
of aptamers in the early 1990s. Aptamers offer inherent advantages over traditional …

Computational tools for aptamer identification and optimization

D Sun, M Sun, J Zhang, X Lin, Y Zhang, F Lin… - TrAC Trends in …, 2022 - Elsevier
Aptamers are single-stranded DNA or RNA oligonucleotides that can selectively bind to a
specific target. They are generally obtained by SELEX, but the procedure is challenging and …

Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives

T Dong, M Wang, J Liu, P Ma, S Pang, W Liu… - Chemical Science, 2023 - pubs.rsc.org
The disastrous spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
has induced severe public healthcare issues and weakened the global economy …

A universal DNA aptamer that recognizes spike proteins of diverse SARS‐CoV‐2 variants of concern

Z Zhang, J Li, J Gu, R Amini, HD Stacey… - … A European Journal, 2022 - Wiley Online Library
We report on a unique DNA aptamer, denoted MSA52, that displays universally high affinity
for the spike proteins of wildtype SARS‐CoV‐2 as well as the Alpha, Beta, Gamma, Epsilon …

Electrochemical aptasensing of SARS-CoV-2 based on triangular prism DNA nanostructures and dumbbell hybridization chain reaction

Y Jiang, X Chen, N Feng, P Miao - Analytical Chemistry, 2022 - ACS Publications
Development of convenient, accurate, and sensitive methods for rapid screening of severe
acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) infection is highly desired. In this …

Design and prediction of aptamers assisted by in silico methods

SJ Lee, J Cho, BH Lee, D Hwang, JW Park - Biomedicines, 2023 - mdpi.com
An aptamer is a single-stranded DNA or RNA that binds to a specific target with high binding
affinity. Aptamers are developed through the process of systematic evolution of ligands by …

Aptamer sandwich assay for the detection of SARS-CoV-2 spike protein antigen

M Svobodova, V Skouridou, M Jauset-Rubio… - ACS …, 2021 - ACS Publications
The novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) emerged at the
end of 2019, resulting in the ongoing COVID-19 pandemic. The high transmissibility of the …