Cells are crowded, but proteins are almost always studied in dilute aqueous buffer. We
review the experimental evidence that crowding affects the equilibrium thermodynamics of …

In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

INTRODUCTION Understanding drug mechanism poses the daunting challenge of
determining the affinity of the drug for all potential targets. Drug target engagement can be …

Protein interactions inside the human body are expected to differ from the situation in vitro.
This is crucial when investigating protein functions or develo** new drugs. In this study …

Biochemical processes take place in heterogeneous and highly volume-occupied or
crowded environments that can considerably influence the reactivity and distribution of …

It has long been axiomatic that a protein's structure determines its function. Intrinsically
disordered proteins (IDPs) and disordered protein regions (IDRs) defy this structure …

Protein misfolding and aggregation, a key contributor to the progression of numerous
neurodegenerative diseases, results in functional deficiencies and the creation of harmful …

Optical imaging has served as a primary method to collect information about biosystems
across scales—from functionalities of tissues to morphological structures of cells and even at …

Biological macromolecules function in highly crowded cellular environments. The structure
and dynamics of proteins and nucleic acids are well characterized in vitro, but in vivo …

One of the grand challenges of biophysical chemistry is to understand the principles that
govern protein misfolding and aggregation, which is a highly complex process that is …