Bioorthogonal chemistry is a set of methods using the chemistry of non-native functional
groups to explore and understand biology in living organisms. In this review, we summarize …

Bioorthogonal chemistry represents a class of high-yielding chemical reactions that proceed
rapidly and selectively in biological environments without side reactions towards …

The emerging inverse electron demand Diels–Alder (IEDDA) reaction stands out from other
bioorthogonal reactions by virtue of its unmatchable kinetics, excellent orthogonality and …

Chemical protein modification has emerged as an invaluable tool for the development of
modified proteins. The complementary use of both genetic and chemical methods has …

Proteins constitute the majority of nature's worker biomolecules. Designed for specific
functions, complex tertiary structures make proteins ideal candidates for analysing natural …

Recently, click chemistry has provided important advances in biomedical research fields.
Particularly, copper-free click chemistry including strain-promoted azide–alkyne …

Over the past decade, remarkable advances have been witnessed in the development of
small-molecule probes. These molecular tools have been widely applied for interrogating …

Over 500 natural and synthetic amino acids have been genetically encoded in the last two
decades. Incorporating these noncanonical amino acids into proteins enables many …

Although proteolysis targeting chimeras (PROTACs) have become promising therapeutic
modalities, important concerns exist about the potential toxicity of the approach owing to …

Traditionally, organic chemical reactions require organic solvents, toxic catalysts, heat, or
high pressure. However, copper‐free click chemistry has been shown to have favorable …