This overview guides both novices and experienced researchers facing challenging targets
to select the most appropriate gene expression system for producing a particular protein. By …

Therapeutic proteins treat many acute and chronic diseases that were until recently
considered untreatable. However, their high development cost keeps them out of reach of …

E. coli plays a substantial role in recombinant protein production. Its importance increased
with the discovery of recombinant DNA technology and the subsequent production of the first …

Fatty acid desaturase catalyzes the desaturation reactions by inserting double bonds into
the fatty acyl chain, producing unsaturated fatty acids, which play a vital part in the synthesis …

Biodegradation using enzyme-based systems is a promising approach to minimize antibiotic
loads in the environment. Aminoglycosides are refractory antibiotics that are generally …

The increased prevalence of diabetes and the growing popularity of non-invasive methods
of recombinant human insulin uptake, such as oral insulin, have increased insulin demand …

Significant advances are being made in the incorporation of enzymes into functional
materials, which leverage their inherent substrate specificity, efficient catalytic activity, and …

Escherichia coli is often used as a factory to produce recombinant proteins. In many cases,
the recombinant protein needs disulfide bonds to fold and function correctly. These proteins …

High‐value heterologous proteins produced in Escherichia coli that contain disulfide bonds
are almost invariably targeted to the periplasm via the Sec pathway as it, among other …

Toxoplasmosis represents a significant public health and veterinary concern due to its
widespread distribution, zoonotic transmission, and potential for severe health impacts in …