High-throughput sequencing, also known as next-generation sequencing (NGS), has
revolutionized genomic research. In recent years, NGS technology has steadily improved …

Highlights•RNA-seq has become the method of choice for bacterial transcriptomics.•
Differential RNA-seq (dRNA-seq) distinguishes primary and processed transcripts.•dRNA …

Abstract The National Center for Biotechnology Information (NCBI) Reference Sequence
(RefSeq) database is a collection of annotated genomic, transcript and protein sequence …

Failure to make adaptive immune responses is a hallmark of aging. Reduced B cell function
leads to poor vaccination efficacy and a high prevalence of infections in the elderly. Here we …

Functional protein-coding small open reading frames (smORFs) are emerging as an
important class of genes. However, the number of translated smORFs in the human genome …

Abstract Background Long noncoding RNAs (lncRNAs) form an abundant class of
transcripts, but the function of the majority of them remains elusive. While it has been shown …

Proprotein convertase subtilisin/kexin type 9 (PCSK9) plays a key role in regulating the
levels of plasma low-density lipoprotein cholesterol (LDL-C). Here, we demonstrate that the …

Bacterial and viral mRNAs are often polycistronic. Akin to alternative splicing, alternative
translation of polycistronic messages is a mechanism to generate protein diversity and …

Background Oxidative phosphorylation (OXPHOS) complexes consist of nuclear and
mitochondrial DNA-encoded subunits. Their biogenesis requires cross-compartment gene …

Abstract The Consensus Coding Sequence (CCDS) project (http://www. ncbi. nlm. nih.
gov/CCDS/) is a collaborative effort to maintain a dataset of protein-coding regions that are …