Immediately following the discovery of the structure of DNA and the semi-conservative
replication of the parental DNA sequence into two new DNA strands, it became apparent …

Studying the dynamics of genome replication in mammalian cells has been historically
challenging. To reveal the location of replication initiation and termination in the human …

Current temporal studies of DNA replication are either low-resolution or require complex cell
synchronisation and/or sorting procedures. Here we introduce Nanotiming, a single …

The transmission and maintenance of genetic information in eukaryotic cells relies on the
faithful duplication of the entire genome. In each round of division, excessive replication …

Dysregulated DNA replication is a cause and a consequence of aneuploidy in cancer, yet
the interplay between copy number alterations (CNAs), replication timing (RT) and cell cycle …

The genome duplication program is affected by multiple factors in vivo, including
developmental cues, genotoxic stress, and aging. Here, we monitored DNA replication …

Eukaryotic DNA replication initiates during S phase from origins that have been licensed in
the preceding G1 phase. Here, we compare ChIP-seq profiles of the licensing factors Orc2 …

DNA replication initiates from replication origins firing throughout S phase. Debate remains
about whether origins are a fixed set of loci, or a loose agglomeration of potential sites used …

The cell division cycle must be strictly regulated during both development and adult
maintenance, and efficient and well-controlled DNA replication is a key event in the cell …

Genomes in eukaryotes normally undergo DNA replication in a choreographed temporal
order, resulting in early and late replicating chromosome compartments. Leishmania, a …