Chemical and enzymatic methods for post-translational protein–protein conjugation
RJ Taylor, MB Geeson, T Journeaux… - Journal of the …, 2022 - ACS Publications
Fusion proteins play an essential role in the biosciences but suffer from several key
limitations, including the requirement for N-to-C terminal ligation, incompatibility of …
limitations, including the requirement for N-to-C terminal ligation, incompatibility of …
Antibody orientation on biosensor surfaces: a minireview
Detection elements play a key role in analyte recognition in biosensors. Therefore, detection
elements with high analyte specificity and binding strength are required. While antibodies …
elements with high analyte specificity and binding strength are required. While antibodies …
A rapid cell-free expression and screening platform for antibody discovery
Antibody discovery is bottlenecked by the individual expression and evaluation of antigen-
specific hits. Here, we address this bottleneck by develo** a workflow combining cell-free …
specific hits. Here, we address this bottleneck by develo** a workflow combining cell-free …
High-throughput preparation methods of crude extract for robust cell-free protein synthesis
Crude extract based cell-free protein synthesis (CFPS) has emerged as a powerful
technology platform for high-throughput protein production and genetic part characterization …
technology platform for high-throughput protein production and genetic part characterization …
Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
The emerging discipline of bacterial glycoengineering has made it possible to produce
designer glycans and glycoconjugates for use as vaccines and therapeutics. Unfortunately …
designer glycans and glycoconjugates for use as vaccines and therapeutics. Unfortunately …
The controlled display of biomolecules on nanoparticles: a challenge suited to bioorthogonal chemistry
Interest in develo** diverse nanoparticle (NP)-biological composite materials continues to
grow almost unabated. This is motivated primarily by the desire to simultaneously exploit the …
grow almost unabated. This is motivated primarily by the desire to simultaneously exploit the …
Noncanonical Amino Acids: Bringing New-to-Nature Functionalities to Biocatalysis
B Brouwer, F Della-Felice, JH Illies… - Chemical …, 2024 - ACS Publications
Biocatalysis has become an important component of modern organic chemistry, presenting
an efficient and environmentally friendly approach to synthetic transformations. Advances in …
an efficient and environmentally friendly approach to synthetic transformations. Advances in …
Development and applications of the copper-catalyzed azide-alkyne cycloaddition (CuAAC) as a bioorthogonal reaction
L Li, Z Zhang - Molecules, 2016 - mdpi.com
The emergence of bioorthogonal reactions has greatly broadened the scope of biomolecule
labeling and detecting. Of all the bioorthogonal reactions that have been developed, the …
labeling and detecting. Of all the bioorthogonal reactions that have been developed, the …
Protein synthesis by ribosomes with tethered subunits
The ribosome is a ribonucleoprotein machine responsible for protein synthesis. In all
kingdoms of life it is composed of two subunits, each built on its own ribosomal RNA (rRNA) …
kingdoms of life it is composed of two subunits, each built on its own ribosomal RNA (rRNA) …
Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development
Immobilized antibody systems are the key to develop efficient diagnostics and separations
tools. In the last decade, developments in the field of biomolecular engineering and …
tools. In the last decade, developments in the field of biomolecular engineering and …