Chemical and enzymatic methods for post-translational protein–protein conjugation

RJ Taylor, MB Geeson, T Journeaux… - Journal of the …, 2022 - ACS Publications
Fusion proteins play an essential role in the biosciences but suffer from several key
limitations, including the requirement for N-to-C terminal ligation, incompatibility of …

Antibody orientation on biosensor surfaces: a minireview

AK Trilling, J Beekwilder, H Zuilhof - Analyst, 2013 - pubs.rsc.org
Detection elements play a key role in analyte recognition in biosensors. Therefore, detection
elements with high analyte specificity and binding strength are required. While antibodies …

A rapid cell-free expression and screening platform for antibody discovery

AC Hunt, B Vögeli, AO Hassan, L Guerrero… - Nature …, 2023 - nature.com
Antibody discovery is bottlenecked by the individual expression and evaluation of antigen-
specific hits. Here, we address this bottleneck by develo** a workflow combining cell-free …

High-throughput preparation methods of crude extract for robust cell-free protein synthesis

YC Kwon, MC Jewett - Scientific reports, 2015 - nature.com
Crude extract based cell-free protein synthesis (CFPS) has emerged as a powerful
technology platform for high-throughput protein production and genetic part characterization …

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

T Jaroentomeechai, JC Stark, A Natarajan… - Nature …, 2018 - nature.com
The emerging discipline of bacterial glycoengineering has made it possible to produce
designer glycans and glycoconjugates for use as vaccines and therapeutics. Unfortunately …

The controlled display of biomolecules on nanoparticles: a challenge suited to bioorthogonal chemistry

WR Algar, DE Prasuhn, MH Stewart… - Bioconjugate …, 2011 - ACS Publications
Interest in develo** diverse nanoparticle (NP)-biological composite materials continues to
grow almost unabated. This is motivated primarily by the desire to simultaneously exploit the …

Noncanonical Amino Acids: Bringing New-to-Nature Functionalities to Biocatalysis

B Brouwer, F Della-Felice, JH Illies… - Chemical …, 2024 - ACS Publications
Biocatalysis has become an important component of modern organic chemistry, presenting
an efficient and environmentally friendly approach to synthetic transformations. Advances in …

Development and applications of the copper-catalyzed azide-alkyne cycloaddition (CuAAC) as a bioorthogonal reaction

L Li, Z Zhang - Molecules, 2016 - mdpi.com
The emergence of bioorthogonal reactions has greatly broadened the scope of biomolecule
labeling and detecting. Of all the bioorthogonal reactions that have been developed, the …

Protein synthesis by ribosomes with tethered subunits

C Orelle, ED Carlson, T Szal, T Florin, MC Jewett… - Nature, 2015 - nature.com
The ribosome is a ribonucleoprotein machine responsible for protein synthesis. In all
kingdoms of life it is composed of two subunits, each built on its own ribosomal RNA (rRNA) …

Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development

M Shen, JF Rusling, CK Dixit - Methods, 2017 - Elsevier
Immobilized antibody systems are the key to develop efficient diagnostics and separations
tools. In the last decade, developments in the field of biomolecular engineering and …