Nitroaromatic compounds (NACs) are extensively used in different industries and are
synthesized in large quantity due to their heavy demand worldwide. The broad use of NACs …

Directed evolution is an important tool for overcoming the limitations of natural enzymes as
biocatalysts. Recent advances have focused on applying directed evolution to a variety of …

Given the immense risk posed by widespread environmental pollution by inorganic and
organic chemicals, novel methods of decontamination and clean-up are required. Owing to …

Bacteria have enormous catabolic potential for remediating wastes; however, the
interactions between bacteria and pollutants are complex and suitable remediation does not …

The field of directed evolution of oxygenases (mono-, di-and epoxygenases) is rapidly
advancing as an increasing number of success stories indicate. A significant number of …

Dinitrotoluene (DNT) is a widely present pollutant in aquatic environments, and its
biodegradation is an economically attractive way to effectively removal. In aquatic …

ABSTRACT Nitrobenzene 1, 2-dioxygenase from Comamonas sp. strain JS765 catalyzes
the initial reaction in nitrobenzene degradation, forming catechol and nitrite. The enzyme …

The large subunit (HycE, 569 amino acids) of Escherichia coli hydrogenase 3 produces
hydrogen from formate via its Ni–Fe-binding site. In this paper, we engineered HycE for …

Dichloronitrobenzenes (DCNB) are intermediates in the production of dichloroanilines,
which are key feedstocks for synthesis of diuron and other herbicides. Although DCNB is a …

3-nitrotoluene dioxygenase (3NTDO) from Diaphorobacter sp. strain DS2 catalyses the
conversion of 3-nitrotoluene (3NT) into a mixture of 3-and 4-methylcatechols with release of …