Charged and polar groups, through forming ion pairs, hydrogen bonds, and other less
specific electrostatic interactions, impart important properties to proteins. Modulation of the …

In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

RNA isoforms influence cell identity and function. However, a comprehensive brain isoform
map was lacking. We analyze single-cell RNA isoforms across brain regions, cell subtypes …

Gene expression is controlled by transcription factors (TFs) that consist of DNA-binding
domains (DBDs) and activation domains (ADs). The DBDs have been well characterized …

NLRP6 is important in host defense by inducing functional outcomes including
inflammasome activation and interferon production. Here, we show that NLRP6 undergoes …

Phase separation of intrinsically disordered proteins (IDPs) is a remarkable feature of living
cells to dynamically control intracellular partitioning. Despite the numerous new IDPs that …

Neuroimmune interactions mediate intercellular communication and underlie critical brain
functions. Microglia, CNS-resident macrophages, modulate the brain through direct physical …

Tripartite-motif protein-56 (TRIM56) positively regulates the induction of type I interferon
response via the TLR3 pathway by enhancing IRF3 activation and depends on its C-terminal …

Many proteins contain large structurally disordered regions or are entirely disordered under
physiological conditions. The functions of these intrinsically disordered proteins (IDPs) often …

We report the de novo design of small (< 20 kDa) and highly soluble synthetic intrinsically
disordered proteins (SynIDPs) that confer solubility to a fusion partner with minimal effect on …