Monoclonal antibodies (mAbs) have become one of the most important classes of
biopharmaceutical products, and they continue to dominate the universe of …

To date, generation of single‐chain fragment variable (scFv) has become an established
technique used to produce a completely functional antigen‐binding fragment in bacterial …

We have mimicked features of immune selection to make human antibodies in bacteria.
Diverse libraries of immunoglobulin heavy (VH) and light (V κ and V λ chain variable (V) …

Antibody fragments of predetermined binding specificity have recently been constructed
from repertoires of antibody V genes, bypassing hybridoma technology and even …

Functional heavy‐chain γ‐immunoglobulins lacking light chains occur naturally in
Camelidae. We now show the feasibility of immunising a dromedary, cloning the repertoire …

A phagemid system was developed for the monovalent display of combinatorial antibody
Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the …

The function and activity of a protein are often modulated by other proteins with which it
interacts. This review is intended as a practical guide to the analysis of such protein-protein …

Filamentous bacteriophage, long and thin filaments that are secreted from the host cells
without killing them, have been an antithesis to the standard view of head-and-tail bacterial …

Practical Handbook of Microbiology, 4th edition provides basic, clear and concise
knowledge and practical information about working with microorganisms. Useful to anyone …

By display of antibody repertoires on the surface of a filamentous bacteriophage and
selection of the phage by binding to antigen, we can mimic immune selection. Recently, by …