Hydrogenases (H2ases) catalyze the reversible oxidation of molecular hydrogen and play a
central role in microbial energy metabolism. Most of these enzymes are found in Archaea …

Nickel is an essential nutrient for selected microorganisms where it participates in a variety
of cellular processes. Many microbes are capable of sensing cellular nickel ion …

Background: Many microorganisms have the ability to either oxidize molecular hydrogen to
generate reducing power or to produce hydrogen in order to remove low-potential electrons …

Crystallographic data on the [NiFe] hydrogenase from Desulfovibrio gigas are presented that
provide new information on the structure and mode of action of its dihydrogen activating …

Enzymes possessing the capacity to oxidize molecular hydrogen have developed
convergently three class of enzymes leading to:[FeFe]-,[NiFe]-, and [FeS]-cluster-free …

Nickel is an essential catalytic cofactor of enzymes found in eubacteria, archaebacteria,
fungi, and plants. 1-6 These enzymes catalyze a diverse array of reactions that include both …

Infrared-spectroscopic studies on the [NiFe]-hydrogenase of Chromatium vinosum-enriched
in 15 N or 13 C, as well as chemical analyses, show that this enzyme contains three non …

Recent research on hydrogenases has been notably motivated by a desire to utilize these
remarkable hydrogen oxidation catalysts in biotechnological applications. Progress in the …

During the era of prebiotic evolution, which culminated in the appearance of cellular life
forms, the earth had a reducing atmosphere. Fueled by volcanic activity and by magmatic …

The high degree of similarity that exists between all the [NiFe] hydrogenase operons and the
near universality of hydrogen metabolism among microorganisms suggest that the microbial …