For a systemically administered therapeutic agent to reach neoplastic cells, it must enter the
blood circulation, cross the vessel wall, move through the extracellular matrix and avoid …

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its fluorescent
homologs from Anthozoa corals have become invaluable tools for in vivo imaging of cells …

The success of fluorescence experiments requires attention to experimental details and an
understanding of the instrumentation. There are also many potential artifacts that can distort …

The subunit number and stoichiometry of membrane-bound proteins are difficult to
determine without disrupting their membrane environment. Here we describe a single …

In the last decade emerging single‐molecule fluorescence‐spectroscopy tools have been
developed and adapted to analyze individual molecules under various conditions. Single …

Since the physical process of fluorescence resonance energy transfer (FRET) was
elucidated more than six decades ago, this peculiar fluorescence phenomenon has turned …

The inheritance of the histone H3 variant CENP-A in nucleosomes at centromeres following
DNA replication is mediated by an epigenetic mechanism. To understand the process of …

Monomeric red fluorescent proteins (mRFPs) have become indispensable tools for studying
protein dynamics, interactions and functions in the cellular environment. Their emission …

The mechanism of the chromophore maturation in members of the green fluorescent protein
(GFP) family such as DsRed and other red fluorescent and chromoproteins was analyzed …

A simple new approach is described and demonstrated for measuring the number of
independent emitters along with the fluorescence intensity, lifetime, and emission …