[HTML][HTML] Manipulating gene expression levels in mammalian cell factories: An outline of synthetic molecular toolboxes to achieve multiplexed control
P Eisenhut, N Marx, G Borsi, M Papež, C Ruggeri… - New …, 2024 - Elsevier
Mammalian cells have developed dedicated molecular mechanisms to tightly control
expression levels of their genes where the specific transcriptomic signature across all genes …
expression levels of their genes where the specific transcriptomic signature across all genes …
Genetic engineering approaches to improve posttranslational modification of biopharmaceuticals in different production platforms
T Amann, V Schmieder… - Biotechnology and …, 2019 - Wiley Online Library
The number of approved biopharmaceuticals, where product quality attributes remain of
major importance, is increasing steadily. Within the available variety of expression hosts, the …
major importance, is increasing steadily. Within the available variety of expression hosts, the …
Modeling apoptosis resistance in CHO cells with CRISPR‐mediated knockouts of Bak1, Bax, and Bok
MA MacDonald, C Barry, T Groves… - Biotechnology and …, 2022 - Wiley Online Library
Chinese hamster ovary (CHO) cells are the primary platform for the production of
biopharmaceuticals. To increase yields, many CHO cell lines have been genetically …
biopharmaceuticals. To increase yields, many CHO cell lines have been genetically …
Let's make it personal: CRISPR tools in manipulating cell death pathways for cancer treatment
Advancements in the CRISPR technology, a game-changer in experimental research, have
revolutionized various fields of life sciences and more profoundly, cancer research. Cell …
revolutionized various fields of life sciences and more profoundly, cancer research. Cell …
Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production
MN Henry, MA MacDonald, CA Orellana… - Biotechnology and …, 2020 - Wiley Online Library
Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of
biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global …
biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global …
[HTML][HTML] Improving recombinant protein production in CHO cells using the CRISPR-Cas system
AK Kalkan, F Palaz, S Sofija, N Elmousa… - Biotechnology …, 2023 - Elsevier
Chinese hamster ovary (CHO) cells are among the most widely used mammalian cell lines
in the biopharmaceutical industry. Therefore, it is not surprising that significant efforts have …
in the biopharmaceutical industry. Therefore, it is not surprising that significant efforts have …
CHO cell productivity improvement by genome-scale modeling and pathway analysis: Application to feed supplements
Effective bioprocess development using Chinese hamster ovary (CHO) cells as hosts is
hampered by the limited understanding of cellular metabolism under process conditions in …
hampered by the limited understanding of cellular metabolism under process conditions in …
Restoration of DNA repair mitigates genome instability and increases productivity of Chinese hamster ovary cells
Chinese hamster ovary (CHO) cells are the primary host for manufacturing of therapeutic
proteins. However, productivity loss is a major problem and is associated with genome …
proteins. However, productivity loss is a major problem and is associated with genome …
Awakening dormant glycosyltransferases in CHO cells with CRISPRa
Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical
industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations …
industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations …
An optimized genome-wide, virus-free CRISPR screen for mammalian cells
Pooled CRISPR screens have been widely applied to mammalian and other organisms to
elucidate the interplay between genes and phenotypes of interest. The most popular method …
elucidate the interplay between genes and phenotypes of interest. The most popular method …