NOTE: In the version of this Review initially published, an author (B. Martin Hallberg) was left
off of the author list. This information has been added to the HTML and PDF versions of the …

This article reviews the development of immobilized-metal affinity chromatography (IMAC)
and describes its most important applications. We provide an overview on the use of IMAC in …

Degeneracy in the genetic code, which enables a single protein to be encoded by a
multitude of synonymous gene sequences, has an important role in regulating protein …

Analytical size-exclusion chromatography (SEC), commonly used for the determination of
the molecular weight of proteins and protein-protein complexes in solution, is a relative …

Protein NMR chemical shifts are highly sensitive to local structure. A robust protocol is
described that exploits this relation for de novo protein structure generation, using as input …

Influenza A viruses are responsible for seasonal epidemics and high mortality pandemics. A
major function of the viral NS1A protein, a virulence factor, is the inhibition of the production …

UNC119 is widely expressed among vertebrates and other phyla. We found that UNC119
recognized the acylated N terminus of the rod photoreceptor transducin α (Tα) subunit and …

Motivation Recombinant protein production is a widely used technique in the biotechnology
and biomedical industries, yet only a quarter of target proteins are soluble and can therefore …

How living organisms create carbon-sulfur bonds during the biosynthesis of critical sulfur-
containing compounds is still poorly understood. The methylthiotransferases MiaB and …

Crystallization is the most serious bottleneck in high-throughput protein-structure
determination by diffraction methods. We have used data mining of the large-scale …