A cell-free biosynthesis platform for modular construction of protein glycosylation pathways

W Kightlinger, KE Duncker, A Ramesh… - Nature …, 2019 - nature.com
Glycosylation plays important roles in cellular function and endows protein therapeutics with
beneficial properties. However, constructing biosynthetic pathways to study and engineer …

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: an update for 2015–2016

DJ Harvey - Mass Spectrometry Reviews, 2021 - Wiley Online Library
This review is the ninth update of the original article published in 1999 on the application of
matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of …

Identification of a second glycoform of the clinically prevalent O1 antigen from Klebsiella pneumoniae

SD Kelly, OG Ovchinnikova, F Müller, M Steffen… - Proceedings of the …, 2023 - pnas.org
Carbapenemase and extended β-lactamase-producing Klebsiella pneumoniae isolates
represent a major health threat, stimulating increasing interest in immunotherapeutic …

Bacillus subtilis EpsA-O: A novel exopolysaccharide structure acting as an efficient adhesive in biofilms

I Dogsa, B Bellich, M Blaznik, C Lagatolla… - npj Biofilms and …, 2024 - nature.com
Extracellular polysaccharides are crucial components for biofilm development. Although
Bacillus subtilis is one of the most characterized Gram-positive biofilm model system, the …

[HTML][HTML] Pyruvate substitutions on glycoconjugates

FF Hager, L Sützl, C Stefanović, M Blaukopf… - International Journal of …, 2019 - mdpi.com
Glycoconjugates are the most diverse biomolecules of life. Mostly located at the cell surface,
they translate into cell-specific “barcodes” and offer a vast repertoire of functions, including …

Functional characterization of enzymatic steps involved in pyruvylation of bacterial secondary cell wall polymer fragments

FF Hager, A López-Guzmán, S Krauter… - Frontiers in …, 2018 - frontiersin.org
Various mechanisms of protein cell surface display have evolved during bacterial evolution.
Several Gram-positive bacteria employ S-layer homology (SLH) domain-mediated sorting of …

Mechanistic insights into Schizosaccharomyces pombe GT-A family protein Pvg3 in the biosynthesis of pyruvylated β1, 3-galactose of N-linked oligosaccharides

T Fukunaga, M Watanabe, Y Nakamichi… - Journal of bioscience …, 2023 - Elsevier
N-linked oligosaccharides in the fission yeast Schizosaccharomyces pombe contain large
amounts of d-galactose (Gal), which mainly comprises α1, 2-and α1, 3-linked Gal except for …

Identification and characterization of a novel β-D-galactosidase that releases pyruvylated galactose

Y Higuchi, H Matsufuji, M Tanuma, T Arakawa, K Mori… - Scientific reports, 2018 - nature.com
Pyruvyl modification of oligosaccharides is widely seen in both prokaryotes and eukaryotes.
Although the biosynthetic mechanisms of pyruvylation have been investigated, enzymes that …

In vivo imaging of fluorescent albumin modified with pyruvylated-human-type complex oligosaccharide reveals sialylation-like biodistribution and kinetics

R Fukuhara, A Ogura, S Yoshinaga, T Fukunaga… - Bioorganic & Medicinal …, 2022 - Elsevier
Both pyruvylation and sialylation onto the terminus of oligosaccharides of N-glycoproteins
seem to be structurally and functionally similar with a property of conferring negative charge …

Characterization of N-and O-linked galactosylated oligosaccharides from fission yeast species

T Fukunaga, N Tanaka, T Furumoto, S Nakakita… - Journal of bioscience …, 2020 - Elsevier
The N-and O-linked oligosaccharides from fission yeast Schizosaccharomyces pombe not
only contain large amounts of d-mannose (Man) but also contain large amounts of d …