Time-resolved cryo-EM using a combination of droplet microfluidics with on-demand jetting
S Torino, M Dhurandhar, A Stroobants, R Claessens… - Nature …, 2023 - nature.com
Single-particle cryogenic electron microscopy (cryo-EM) allows reconstruction of high-
resolution structures of proteins in different conformations. Protein function often involves …
resolution structures of proteins in different conformations. Protein function often involves …
Droplet microfluidics: from proof-of-concept to real-world utility?
Droplet microfluidics constitutes a diverse and practical tool set that enables chemical and
biological experiments to be performed at high speed and with enhanced efficiency when …
biological experiments to be performed at high speed and with enhanced efficiency when …
Single-cell RT-PCR in microfluidic droplets with integrated chemical lysis
Droplet microfluidics can identify and sort cells using digital reverse transcription
polymerase chain reaction (RT-PCR) signals from individual cells. However, current …
polymerase chain reaction (RT-PCR) signals from individual cells. However, current …
Easy-to-operate co-flow step emulsification device for droplet digital polymerase chain reaction
C Wei, C Yu, S Li, J Meng, T Li, J Cheng… - Analytical …, 2022 - ACS Publications
Digital polymerase chain reaction (PCR) plays important roles in the detection and
quantification of nucleic acid targets, while there still remain challenges including high cost …
quantification of nucleic acid targets, while there still remain challenges including high cost …
Activity-based DNA-encoded library screening
WG Cochrane, ML Malone, VQ Dang… - ACS combinatorial …, 2019 - ACS Publications
Robotic high-throughput compound screening (HTS) and, increasingly, DNA-encoded
library (DEL) screening are driving bioactive chemical matter discovery in the postgenomic …
library (DEL) screening are driving bioactive chemical matter discovery in the postgenomic …
Microfluidic screening of antibiotic susceptibility at a single-cell level shows the inoculum effect of cefotaxime on E. coli
Measurement of antibiotic susceptibility at the level of single cells is important as it reveals
the concentration of an antibiotic that leads to drug resistance in bacterial strains. To date …
the concentration of an antibiotic that leads to drug resistance in bacterial strains. To date …
Multifunctional regulation of 3D cell-laden microsphere culture on an integrated microfluidic device
Three-dimensional (3D) hydrogel microspheres have aroused increasing attention as an in
vitro cell culture model. Yet the preservation of cells' original biological properties has been …
vitro cell culture model. Yet the preservation of cells' original biological properties has been …
The effect of microfluidic chip geometry on droplet clustering in a high throughput droplet incubation platform for single-cell analysis
High-throughput droplet incubation is an integral part of various lab-on-chip platforms.
Packing droplets efficiently before sending them to the incubation region is essential. For …
Packing droplets efficiently before sending them to the incubation region is essential. For …
Tuna-step: tunable parallelized step emulsification for the generation of droplets with dynamic volume control to 3D print functionally graded porous materials
We present tuna-step, a novel microfluidic module based on step emulsification that allows
for reliable generation of droplets of different sizes. Until now, sizes of droplets generated …
for reliable generation of droplets of different sizes. Until now, sizes of droplets generated …
Electrorheology of a dilute emulsion of surfactant-covered drops
We investigate the effects of surfactant coating on a deformable viscous drop under the
combined action of shear flow and a uniform electric field. Employing a comprehensive three …
combined action of shear flow and a uniform electric field. Employing a comprehensive three …