Structural and chemical biology of terpenoid cyclases

DW Christianson - Chemical reviews, 2017 - ACS Publications
The year 2017 marks the twentieth anniversary of terpenoid cyclase structural biology: a trio
of terpenoid cyclase structures reported together in 1997 were the first to set the foundation …

Metabolic engineering of Escherichia coli for natural product biosynthesis

D Yang, SY Park, YS Park, H Eun, SY Lee - Trends in Biotechnology, 2020 - cell.com
Natural products are widely employed in our daily lives as food additives, pharmaceuticals,
nutraceuticals, and cosmetic ingredients, among others. However, their supply has often …

Synthetic biology identifies the minimal gene set required for paclitaxel biosynthesis in a plant chassis

Y Zhang, L Wiese, H Fang, S Alseekh, LP de Souza… - Molecular Plant, 2023 - cell.com
The diterpenoid paclitaxel (Taxol) is a chemotherapy medication widely used as a first-line
treatment against several types of solid cancers. The supply of paclitaxel from natural …

Reconstitution of early paclitaxel biosynthetic network

JCT Liu, R De La Peña, C Tocol, ES Sattely - Nature Communications, 2024 - nature.com
Paclitaxel is an anticancer therapeutic produced by the yew tree. Over the last two decades,
a significant bottleneck in the reconstitution of early paclitaxel biosynthesis has been the …

Protein engineering for improving and diversifying natural product biosynthesis

C Li, R Zhang, J Wang, LM Wilson, Y Yan - Trends in biotechnology, 2020 - cell.com
Proteins found in nature have traditionally been the most frequently used biocatalysts to
produce numerous natural products ranging from commodity chemicals to pharmaceuticals …

Current perspectives on taxanes: Focus on their bioactivity, delivery and combination therapy

J Škubník, V Pavlíčková, T Ruml, S Rimpelová - Plants, 2021 - mdpi.com
Taxanes, mainly paclitaxel and docetaxel, the microtubule stabilizers, have been well known
for being the first-line therapy for breast cancer for more than the last thirty years. Moreover …

Identifying and engineering the ideal microbial terpenoid production host

S Moser, H Pichler - Applied Microbiology and Biotechnology, 2019 - Springer
More than 70,000 different terpenoid structures are known so far; many of them offer highly
interesting applications as pharmaceuticals, flavors and fragrances, or biofuels. Extraction of …

Unraveling the catalytic mechanism of taxadiene-5α-hydroxylase from crystallography and computational analyses

X Song, Q Wang, X Zhu, W Fang, X Liu, C Shi… - ACS …, 2024 - ACS Publications
Paclitaxel is a famous chemotherapeutic agent, but its microbial production poses a long-
standing challenge due to its poor product selectivity. Taxadiene-5α-hydroxylase …

Recent advances of metabolic engineering strategies in natural isoprenoid production using cell factories

M Li, F Hou, T Wu, X Jiang, F Li, H Liu, M **an… - Natural product …, 2020 - pubs.rsc.org
Covering: up to 2019 As abundant natural products, isoprenoids have many useful industrial
applications in the manufacturing of drugs, fragrances, food additives, colorants, rubber and …

Hydroxylases involved in terpenoid biosynthesis: a review

Z Zhang, QY Wu, Y Ge, ZY Huang, R Hong, A Li… - Bioresources and …, 2023 - Springer
Terpenoids are pervasive in nature and display an immense structural diversity. As the
largest category of plant secondary metabolites, terpenoids have important socioeconomic …