The design of new protein folds represents a grand challenge for synthetic, chemical and
structural biology. Due to the good understanding of the principles governing its pairing …

Biomaterials made by living systems, while very diverse, generally share similar
organization: different lineages of cells are precisely patterned within a matrix to create a …

T cells expressing chimeric antigen receptors (CARs) are promising cancer therapeutic
agents, with the prospect of becoming the ultimate smart cancer therapeutics. To expand the …

Many intrinsically disordered proteins self-assemble into liquid droplets that function as
membraneless organelles. Because of their biological importance and ability to colocalize …

Subcellular compartmentalization of macromolecules increases flux and prevents inhibitory
interactions to control biochemical reactions. Inspired by this functionality, we sought to build …

Genetic circuits perform computational operations based on interactions between freely
diffusing molecules within a cell. When transcription factors are combined to build a circuit …

Specificity of interactions between two DNA strands, or between protein and DNA, is often
achieved by varying bases or side chains coming off the DNA or protein backbone—for …

Protein–protein interactions (PPIs) regulate many cellular processes and engineered PPIs
have cell and gene therapy applications. Here, we introduce massively parallel PPI …

Artificial multienzyme scaffolds are being developed for in vitro cascaded biocatalytic activity
and, in particular, accessing substrate channeling. This review covers progress in this field …

Protein engineering, chemical biology, and synthetic biology would benefit from toolkits of
peptide and protein components that could be exchanged reliably between systems while …