Biobanking of vegetable genetic resources by in vitro conservation and cryopreservation

C Ruta, M Lambardi, EA Ozudogru - Biodiversity and Conservation, 2020 - Springer
Today, application of in vitro culture by means of slow growth storage of shoot cultures and
cryopreservation of organs, tissues and cells in liquid nitrogen presents a remarkable …

Cryopreservation of shoot tips of recalcitrant and tropical species: Advances and strategies

MN Normah, N Sulong, BM Reed - Cryobiology, 2019 - Elsevier
There is a pressing need for practical and successful conservation efforts to establish long-
term germplasm collections of recalcitrant and tropical species, given the challenge and …

V-cryo-plate procedure as an effective protocol for cryobanks: case study of mint cryopreservation

S Yamamoto, T Rafique, K Fukui, K Sekizawa… - …, 2012 - ingentaconnect.com
A vitrification procedure using aluminium cryo-plates (V-Cryo-plate procedure) was
successfully developed and adjusted for in vitro-grown mint (Mentha spp.) shoot tips. Shoots …

In Vitro methods and the challenge of exceptional species for target 8 of the global strategy for plant conservation1

VC Pence - Annals of the Missouri Botanical Garden, 2013 - BioOne
For the majority of plant species of conservation concern, seed banking and traditional
propagation methods are the most efficient ways of meeting the ex situ and recovery …

Cryopreservation of in vitro-grown shoot-tips of Rabdosia rubescens by encapsulation-dehydration and evaluation of their genetic stability

PF Ai, LP Lu, JJ Song - Plant Cell, Tissue and Organ Culture (PCTOC), 2012 - Springer
Shoot-tips of Rabdosia rubescens, excised from in vitro-grown proliferating shoots that were
cold-hardened at 5° C for 3 weeks, were encapsulated in alginate beads. Subsequently …

Controlled rate cooling

BM Reed, E Uchendu - Plant cryopreservation: a practical guide, 2008 - Springer
Several conditioning strategies are used to prepare plant materials for controlled rate
cooling. They include osmotic adjustments, cold acclimation periods (Dumet et al. 1993; …

Tracking Permeation of Dimethyl Sulfoxide (DMSO) in Mentha × piperita Shoot Tips Using Coherent Raman Microscopy

HD Kreckel, FMD Samuels, R Bonnart, GM Volk… - Plants, 2023 - mdpi.com
Cryopreservation has emerged as a low-maintenance, cost-effective solution for the long-
term preservation of vegetatively propagated crops. Shoot tip cryopreservation often makes …

[PDF][PDF] Cryopreservation of Mentha piperita L. germplasm and confirmation of genetic stability after cryo-storage.

E Ogur, N Adanacioglu, S Galatali… - JAPS: Journal of …, 2023 - thejaps.org.pk
Peppermint is an important aromatic-medicinal plant species and it has valuable essential
oil contents such as menthol, linalool and limonene. The main purpose of the current study …

Cryopreservation of Thymus cariensis and T. vulgaris shoot tips: comparison of three vitrification-based methods

EA Ozudogru, E Kaya - CryoLetters, 2012 - ingentaconnect.com
Thymus is an important genus of the Lamiaceae family, comprising more than 400 perennial
aromatic thyme species, which are used extensively for medicinal and culinary purposes …

Influence of the cryopreservation technique, recovery medium and genotype on genetic stability of mint cryopreserved shoot tips

C Martín, C Kremer, I González… - Plant Cell, Tissue and …, 2015 - Springer
This study compared the effect of the cryopreservation protocol, the genotype and the plant
growth regulators used in the recovery media on the genetic stability of mint shoot tips …