Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Over the past 20 years, protein engineering has been extensively used to improve and
modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting …

Effective intracellular delivery of therapeutic proteins can potentially treat a wide array of
diseases. However, efficient delivery of functional proteins across the cell membrane …

The slow maturation time of fluorescent proteins (FPs) limits the temporal accuracy of
measurements of rapid processes such as gene expression dynamics and effectively …

The spatial organization of chromatin critically affects genome function. Recent chromosome-
conformation-capture studies have revealed topologically associating domains (TADs) as a …

Once described as mere “bags of enzymes,” bacterial cells are in fact highly organized, with
many macromolecules exhibiting nonuniform localization patterns. Yet the physical and …

Quantitative fluorescence and superresolution microscopy are often limited by insufficient
data quality or artifacts. In this context, it is essential to have biologically relevant control …

Sulfoxide-bridged dimeric BODIPYs were developed as a new class of long-wavelength
photoconvertible fluorophores. Upon visible-light irradiation, a sulfoxide moiety was …

Cell dormancy is a widespread mechanism used by bacteria to evade environmental
threats, including antibiotics. Here we monitored bacterial antibiotic tolerance and regrowth …

Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …