Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization

JM Bolivar, JM Woodley… - Chemical Society …, 2022‏ - pubs.rsc.org
Enzyme immobilization has been develo** since the 1960s and although many industrial
biocatalytic processes use the technology to improve enzyme performance, still today we …

[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

RC Rodrigues, Á Berenguer-Murcia… - Biotechnology …, 2021‏ - Elsevier
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …

Enzyme co-immobilization: Always the biocatalyst designers' choice… or not?

S Arana-Peña, D Carballares… - Biotechnology …, 2021‏ - Elsevier
The increasing relevance of cascade reactions in biocatalysis has sparked a great interest
for enzyme co-immobilization. Enzyme co-immobilization allows access to some kinetic …

Integrating enzyme immobilization and protein engineering: An alternative path for the development of novel and improved industrial biocatalysts

C Bernal, K Rodriguez, R Martinez - Biotechnology Advances, 2018‏ - Elsevier
Enzyme immobilization often achieves reusable biocatalysts with improved operational
stability and solvent resistance. However, these modifications are generally associated with …

Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres… - Biotechnology …, 2015‏ - Elsevier
In this review, we detail the efforts performed to couple the purification and the
immobilization of industrial enzymes in a single step. The use of antibodies, the …

Recent applications and future prospects of magnetic biocatalysts

RLF Melo, MB Sales, V de Castro Bizerra… - International Journal of …, 2023‏ - Elsevier
Magnetic biocatalysts combine magnetic properties with the catalytic activity of enzymes,
achieving easy recovery and reuse in biotechnological processes. Lipases immobilized by …

Importance of the support properties for immobilization or purification of enzymes

JCS Santos, O Barbosa, C Ortiz… - …, 2015‏ - Wiley Online Library
Immobilization and purification of enzymes are usual requirements for their industrial use.
Both purification and immobilization have a common factor: they use a solid activated …

Developments in support materials for immobilization of oxidoreductases: A comprehensive review

J Zdarta, AS Meyer, T Jesionowski, M Pinelo - Advances in colloid and …, 2018‏ - Elsevier
Bioremediation, a biologically mediated transformation or degradation of persistent
chemicals into nonhazardous or less-hazardous substances, has been recognized as a key …

[HTML][HTML] A review of biomimetic surface functionalization for bone-integrating orthopedic implants: Mechanisms, current approaches, and future directions

C Stewart, B Akhavan, SG Wise, MMM Bilek - Progress in Materials …, 2019‏ - Elsevier
Orthopedic implants are increasing in global prevalence, with hundreds of thousands of
operations performed annually. However, a significant proportion of these operations …

Glutaraldehyde in bio-catalysts design: A useful crosslinker and a versatile tool in enzyme immobilization

O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres… - Rsc Advances, 2014‏ - pubs.rsc.org
Glutaraldehyde is one of the most widely used reagents in the design of biocatalysts. It is a
powerful crosslinker, able to react with itself, with the advantages that this may bring forth. In …