Membrane proteins and membrane proteomics

S Tan, HT Tan, MCM Chung - Proteomics, 2008 - Wiley Online Library
Biological membranes form an essential barrier between living cells and their external
environments, as well as serve to compartmentalize intracellular organelles within …

Integral membrane proteins in proteomics. How to break open the black box?

O Vit, J Petrak - Journal of proteomics, 2017 - Elsevier
Integral membrane proteins (IMPs) are coded by 20–30% of human genes and execute
important functions–transmembrane transport, signal transduction, cell-cell communication …

Genomic and proteomic characterization of “Candidatus Nitrosopelagicus brevis”: An ammonia-oxidizing archaeon from the open ocean

AE Santoro, CL Dupont, RA Richter, MT Craig… - Proceedings of the …, 2015 - pnas.org
Thaumarchaeota are among the most abundant microbial cells in the ocean, but difficulty in
cultivating marine Thaumarchaeota has hindered investigation into the physiological and …

The Transcriptome and Proteome of the Diatom Thalassiosira pseudonana Reveal a Diverse Phosphorus Stress Response

ST Dyhrman, BD Jenkins, TA Rynearson, MA Saito… - PloS one, 2012 - journals.plos.org
Phosphorus (P) is a critical driver of phytoplankton growth and ecosystem function in the
ocean. Diatoms are an abundant class of marine phytoplankton that are responsible for …

Multiplexed absolute quantification for proteomics using concatenated signature peptides encoded by QconCAT genes

JM Pratt, DM Simpson, MK Doherty, J Rivers… - Nature protocols, 2006 - nature.com
An important area of proteomics involves the need for quantification, whether relative or
absolute. Many methods now exist for relative quantification, but to support biomarker …

Dinoflagellates alter their carbon and nutrient metabolic strategies across environmental gradients in the central Pacific Ocean

NR Cohen, MR McIlvin, DM Moran, NA Held… - Nature …, 2021 - nature.com
Marine microeukaryotes play a fundamental role in biogeochemical cycling through the
transfer of energy to higher trophic levels and vertical carbon transport. Despite their global …

[HTML][HTML] Quantification of extracellular matrix proteins from a rat lung scaffold to provide a molecular readout for tissue engineering

RC Hill, EA Calle, M Dzieciatkowska… - Molecular & Cellular …, 2015 - Elsevier
The use of extracellular matrix (ECM) 1 scaffolds, derived from decellularized tissues for
engineered organ generation, holds enormous potential in the field of regenerative …

Multiple nutrient stresses at intersecting Pacific Ocean biomes detected by protein biomarkers

MA Saito, MR McIlvin, DM Moran, TJ Goepfert… - Science, 2014 - science.org
Marine primary productivity is strongly influenced by the scarcity of required nutrients, yet our
understanding of these nutrient limitations is informed by experimental observations with …

Identification of Protein Components in in vivo Human Acquired Enamel Pellicle Using LC−ESI−MS/MS

WL Siqueira, W Zhang, EJ Helmerhorst… - Journal of proteome …, 2007 - ACS Publications
The acquired enamel pellicle is a thin protein film forming upon exposure of tooth enamel
surfaces to saliva. The structural analysis of this integument relies on efficient pellicle …

Gel‐aided sample preparation (GASP)—A simplified method for gel‐assisted proteomic sample generation from protein extracts and intact cells

R Fischer, BM Kessler - Proteomics, 2015 - Wiley Online Library
We describe a “gel‐assisted” proteomic sample preparation method for MS analysis.
Solubilized protein extracts or intact cells are copolymerized with acrylamide, facilitating …