Glucansucrases: three-dimensional structures, reactions, mechanism, α-glucan analysis and their implications in biotechnology and food applications

H Leemhuis, T Pijning, JM Dobruchowska… - Journal of …, 2013 - Elsevier
Glucansucrases are extracellular enzymes that synthesize a wide variety of α-glucan
polymers and oligosaccharides, such as dextran. These carbohydrates have found …

Biotechnological potential of novel glycoside hydrolase family 70 enzymes synthesizing α-glucans from starch and sucrose

J Gangoiti, T Pijning, L Dijkhuizen - Biotechnology advances, 2018 - Elsevier
Transglucosidases belonging to the glycoside hydrolase (GH) family 70 are promising
enzymatic tools for the synthesis of α-glucans with defined structures from renewable …

Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutans

K Ito, S Ito, T Shimamura, S Weyand… - Journal of molecular …, 2011 - Elsevier
Glucansucrase (GSase) from Streptococcus mutans is an essential agent in dental caries
pathogenesis. Here, we report the crystal structure of S. mutans glycosyltransferase (GTF …

Lactic acid bacteria-derived α-glucans: From enzymatic synthesis to miscellaneous applications

Z Chen, D Ni, W Zhang, T Stressler, W Mu - Biotechnology Advances, 2021 - Elsevier
Lactic acid bacteria (LAB) are capable of producing a variety of exopolysaccharide α-
glucans, such as dextran, mutan, reuteran, and alternan. Their structural diversity allows …

Recent developments in the enzymatic O-glycosylation of flavonoids

B Hofer - Applied microbiology and biotechnology, 2016 - Springer
The glycosylation of bioactive compounds, such as flavonoids, is of particular relevance, as
it modulates many of their pharmacokinetic parameters. This article reviews the literature …

Structure–function relationships of family GH70 glucansucrase and 4, 6-α-glucanotransferase enzymes, and their evolutionary relationships with family GH13 …

X Meng, J Gangoiti, Y Bai, T Pijning… - Cellular and Molecular …, 2016 - Springer
Lactic acid bacteria (LAB) are known to produce large amounts of α-glucan
exopolysaccharides. Family GH70 glucansucrase (GS) enzymes catalyze the synthesis of …

Bacterial α-glucan and branching sucrases from GH70 family: Discovery, structure–function relationship studies and engineering

M Molina, G Cioci, C Moulis, E Séverac… - Microorganisms, 2021 - mdpi.com
Glucansucrases and branching sucrases are classified in the family 70 of glycoside
hydrolases. They are produced by lactic acid bacteria occupying very diverse ecological …

Enzymatic synthesis of artificial polysaccharides

PJ Smith, ME Ortiz-Soto, C Roth… - ACS Sustainable …, 2020 - ACS Publications
Polysaccharides are the most important renewable polymers on Earth and hold an
enormous potential for the production of ecofriendly functional materials. In addition to being …

4, 6-α-Glucanotransferase, a novel enzyme that structurally and functionally provides an evolutionary link between glycoside hydrolase enzyme families 13 and 70

S Kralj, P Grijpstra, SS van Leeuwen… - Applied and …, 2011 - Am Soc Microbiol
Lactobacillus reuteri 121 uses the glucosyltransferase A (GTFA) enzyme to convert sucrose
into large amounts of the α-d-glucan reuteran, an exopolysaccharide. Upstream of gtfA lies …

NMR of glycans: shedding new light on old problems

MD Battistel, HF Azurmendi, B Yu… - Progress in nuclear …, 2014 - Elsevier
The diversity in molecular arrangements and dynamics displayed by glycans renders
traditional NMR strategies, employed for proteins and nucleic acids, insufficient. Because of …