Functional organic materials with enhanced two‐photon absorption lead to new
technologies in the fields of chemistry, biology, and photonics. In this article we review …

We demonstrate the use of saturated excitation in confocal fluorescence microscopy to
improve the spatial resolution. In the proposed technique, we modulate the excitation …

Fluorescence correlation spectroscopy (FCS) is a minimally invasive real-time fluorescence
technique capable of detecting single molecules in vitro and in situ. By recording and …

Multifocal multiphoton microscopy (MMM) enhances imaging speed by parallelization. It is
not well understood why the imaging depth of MMM is significantly shorter than conventional …

Three-photon wide-field depth-resolved excitation is used to overcome some of the
limitations in conventional point-scanning two-and three-photon microscopy. Excitation of …

This report covers the two-photon activation and excitation properties of the PA-GFP, a
photoactivatable variant of the Aequorea victoria green fluorescent protein in the spectral …

" Alberto Diaspro has been choreographing light's dance for over 20 years, and in
Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a …

We report a multifocal multiphoton time-correlated single photon counting (TCSPC)
fluorescence lifetime imaging (FLIM) microscope system that uses a 16 channel multi-anode …

Fluorescence fluctuation spectroscopy has become an important measurement tool for
investigating molecular dynamics, molecular interactions, and chemical kinetics in biological …

Line-scanning temporal focusing microscopy (LineTFM) is capable of imaging biological
samples more than 10 times faster than two-photon laser point-scanning microscopy …