In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

After cell entry, human immunodeficiency virus type 1 (HIV-1) replication involves reverse
transcription of the RNA genome, nuclear import of the subviral complex without nuclear …

INTRODUCTION The eukaryotic nucleus protects the genome and is enclosed by the two
membranes of the nuclear envelope. Nuclear pore complexes (NPCs) perforate the nuclear …

INTRODUCTION The nucleus harbors and protects the genetic information in eukaryotes. It
is surrounded by the nuclear envelope, which separates the nucleoplasm from the cytosol …

Nuclear pore complexes (NPCs) create large conduits for cargo transport between the
nucleus and cytoplasm across the nuclear envelope (NE),–. These multi-megadalton …

INTRODUCTION Cytoplasmic face of the human NPC. Near-atomic composite structure of
the NPC generated by docking high-resolution crystal structures into a cryo‑ET …

HIV-1 infection requires nuclear entry of the viral genome. Previous evidence suggests that
this entry proceeds through nuclear pore complexes (NPCs), with the 120× 60 nm capsid …

INTRODUCTION In eukaryotic cells, the selective bidirectional transport of macromolecules
between the nucleus and cytoplasm occurs through the nuclear pore complex (NPC) …

HIV-1 replication commences inside the cone-shaped viral capsid, but timing, localization,
and mechanism of uncoating are under debate. We adapted a strategy to visualize …

Of the 13 known independent zoonoses of simian immunodeficiency viruses to humans,
only one, leading to human immunodeficiency virus (HIV) type 1 (M) has become pandemic …