Cell-free gene expression: an expanded repertoire of applications
Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …
It has been used for more than 50 years across the life sciences as a foundational research …
Artificial cells: synthetic compartments with life-like functionality and adaptivity
Conspectus Cells are highly advanced microreactors that form the basis of all life. Their
fascinating complexity has inspired scientists to create analogs from synthetic and natural …
fascinating complexity has inspired scientists to create analogs from synthetic and natural …
Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale
Many industrial chemicals that are produced from fossil resources could be manufactured
more sustainably through fermentation. Here we describe the development of a carbon …
more sustainably through fermentation. Here we describe the development of a carbon …
A user's guide to cell-free protein synthesis
Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities
for protein expression, metabolic engineering, therapeutic development, education, and …
for protein expression, metabolic engineering, therapeutic development, education, and …
De novo design of protein logic gates
The design of modular protein logic for regulating protein function at the posttranscriptional
level is a challenge for synthetic biology. Here, we describe the design of two-input AND …
level is a challenge for synthetic biology. Here, we describe the design of two-input AND …
In vitro prototy** and rapid optimization of biosynthetic enzymes for cell design
The design and optimization of biosynthetic pathways for industrially relevant, non-model
organisms is challenging due to transformation idiosyncrasies, reduced numbers of …
organisms is challenging due to transformation idiosyncrasies, reduced numbers of …
Engineering growth factors for regenerative medicine applications
Growth factors are important morphogenetic proteins that instruct cell behavior and guide
tissue repair and renewal. Although their therapeutic potential holds great promise in …
tissue repair and renewal. Although their therapeutic potential holds great promise in …
Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs)
It has long been axiomatic that a protein's structure determines its function. Intrinsically
disordered proteins (IDPs) and disordered protein regions (IDRs) defy this structure …
disordered proteins (IDPs) and disordered protein regions (IDRs) defy this structure …
Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
The emerging discipline of bacterial glycoengineering has made it possible to produce
designer glycans and glycoconjugates for use as vaccines and therapeutics. Unfortunately …
designer glycans and glycoconjugates for use as vaccines and therapeutics. Unfortunately …
Cell-free protein synthesis: applications come of age
Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the
growing demand for simple and efficient protein production. While used for decades as a …
growing demand for simple and efficient protein production. While used for decades as a …