Resistance threatens to render antibiotics—which are essential for modern medicine—
ineffective, thus posing a threat to human health. The discovery of novel classes of …

Randomly barcoded transposon mutant libraries are powerful tools for studying gene
function and organization, assessing gene essentiality and pathways, discovering potential …

Molecular profiling of small molecules offers invaluable insights into the function of
compounds and allows for hypothesis generation about small-molecule direct targets and …

Antisense peptide nucleic acids (PNAs) that target mRNAs of essential bacterial genes
exhibit specific bactericidal effects in several microbial species, but our mechanistic …

Transcription termination factor ρ is a hexameric, RNA-dependent NTPase that can adopt
active closed-ring and inactive open-ring conformations. The Sm-like protein Rof, a homolog …

The critical role of the intestinal microbiota in human health and disease is well recognized.
Nevertheless, there are still large gaps in our understanding of the functions and …

Highlights•The identification of novel drug targets is a key step in drug innovation.•Essential
genes are vital for organism survival and are promising drug targets.•We describe in detail …

In this study, a 14-gene edited Pseudomonas putida KT2440 strain for heterologous
indigoidine production was examined using three distinct omic datasets. Transcriptomic data …

Gram-negative bacteria can antagonize neighboring microbes using a type VI secretion
system (T6SS) to deliver toxins that target different essential cellular features. Despite the …

Essential genes encode the processes that are necessary for life. Until recently, commonly
applied binary classifications left no space between essential and non-essential genes. In …