Unlocking the potential of the human genome with RNA interference
The discovery of RNA interference (RNAi) may well be one of the transforming events in
biology in the past decade. RNAi can result in gene silencing or even in the expulsion of …
biology in the past decade. RNAi can result in gene silencing or even in the expulsion of …
siRNAs: applications in functional genomics and potential as therapeutics
Y Dorsett, T Tuschl - Nature reviews Drug discovery, 2004 - nature.com
Molecules that can specifically silence gene expression are powerful research tools. Much
effort has been put into the development of such molecules and has resulted in the creation …
effort has been put into the development of such molecules and has resulted in the creation …
Second-generation shRNA libraries covering the mouse and human genomes
JM Silva, MZ Li, K Chang, W Ge, MC Golding… - Nature …, 2005 - nature.com
Loss-of-function phenotypes often hold the key to understanding the connections and
biological functions of biochemical pathways. We and others previously constructed libraries …
biological functions of biochemical pathways. We and others previously constructed libraries …
Reconstruction of cellular signalling networks and analysis of their properties
The study of cellular signalling over the past 20 years and the advent of high-throughput
technologies are enabling the reconstruction of large-scale signalling networks. After careful …
technologies are enabling the reconstruction of large-scale signalling networks. After careful …
Improved targeting of miRNA with antisense oligonucleotides
S Davis, B Lollo, S Freier, C Esau - Nucleic acids research, 2006 - academic.oup.com
MicroRNAs (miRNAs) are a class of 20–24 nt noncoding RNAs that regulate target mRNAs
post-transcriptionally by binding with imperfect complementarity in the 3′-untranslated …
post-transcriptionally by binding with imperfect complementarity in the 3′-untranslated …
RNA interference: from gene silencing to gene-specific therapeutics
RKM Leung, PA Whittaker - Pharmacology & therapeutics, 2005 - Elsevier
In the past 4 years, RNA interference (RNAi) has become widely used as an experimental
tool to analyse the function of mammalian genes, both in vitro and in vivo. By harnessing an …
tool to analyse the function of mammalian genes, both in vitro and in vivo. By harnessing an …
Prospects of RNA interference therapy for cancer
RNA interference (RNAi) is a powerful gene-silencing process that holds great promise in
the field of cancer therapy. The discovery of RNAi has generated enthusiasm within the …
the field of cancer therapy. The discovery of RNAi has generated enthusiasm within the …
An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division
RNA interference (RNAi) is an evolutionarily conserved defence mechanism whereby genes
are specifically silenced through degradation of messenger RNAs; this process is mediated …
are specifically silenced through degradation of messenger RNAs; this process is mediated …
siRNA-mediated off-target gene silencing triggered by a 7 nt complementation
X Lin, X Ruan, MG Anderson, JA McDowell… - Nucleic acids …, 2005 - academic.oup.com
A growing body of evidence suggests that siRNA could generate off-target effects through
different mechanisms. However, the full impact of off-target gene regulation on phenotypic …
different mechanisms. However, the full impact of off-target gene regulation on phenotypic …
Improving the efficiency of RNA interference in mammals
V Mittal - Nature reviews genetics, 2004 - nature.com
RNA interference (RNAi) has been very successfully applied as a gene-silencing technology
in both plants and invertebrates, but many practical obstacles need to be overcome before it …
in both plants and invertebrates, but many practical obstacles need to be overcome before it …