[HTML][HTML] N-linked protein glycosylation in the ER

M Aebi - Biochimica et Biophysica Acta (BBA)-Molecular Cell …, 2013 - Elsevier
N-linked protein glycosylation in the endoplasmic reticulum (ER) is a conserved two phase
process in eukaryotic cells. It involves the assembly of an oligosaccharide on a lipid carrier …

Susceptibility of protein therapeutics to spontaneous chemical modifications by oxidation, cyclization, and elimination reactions

L Grassi, C Cabrele - Amino Acids, 2019 - Springer
Peptides and proteins are preponderantly emerging in the drug market, as shown by the
increasing number of biopharmaceutics already approved or under development …

Posttranslational protein modifications in plant metabolism

G Friso, KJ van Wijk - Plant physiology, 2015 - academic.oup.com
Posttranslational modifications (PTM s) of proteins greatly expand proteome diversity,
increase functionality, and allow for rapid responses, all at relatively low costs for the cell …

Heterogeneity of monoclonal antibodies revealed by charge-sensitive methods

J Vlasak, R Ionescu - Current pharmaceutical biotechnology, 2008 - ingentaconnect.com
The expanding field of monoclonal antibody-based pharmaceuticals has triggered
increased interest in analytical characterization of these large proteins and in understanding …

Middle-down analysis of monoclonal antibodies with electron transfer dissociation orbitrap fourier transform mass spectrometry

L Fornelli, D Ayoub, K Aizikov, A Beck… - Analytical …, 2014 - ACS Publications
The rapid growth of approved biotherapeutics, eg, monoclonal antibodies or
immunoglobulins G (IgGs), demands improved techniques for their quality control …

Proteins in art, archaeology, and paleontology: from detection to identification

S Dallongeville, N Garnier, C Rolando… - Chemical …, 2016 - ACS Publications
Paleontology, archeology, and art history provide crucial information for the understanding
of human evolution throughout ages and its habits. Past animal and plant species are also …

Metamorphism in TDP-43 prion-like domain determines chaperone recognition

J Carrasco, R Antón, A Valbuena… - Nature …, 2023 - nature.com
The RNA binding protein TDP-43 forms cytoplasmic inclusions via its C-terminal prion-like
domain in several neurodegenerative diseases. Aberrant TDP-43 aggregation arises upon …

High-throughput multi-attribute analysis of antibody-drug conjugates enabled by trapped ion mobility spectrometry and top-down mass spectrometry

EJ Larson, DS Roberts, JA Melby, KM Buck… - Analytical …, 2021 - ACS Publications
Antibody-drug conjugates (ADCs) are one of the fastest growing classes of anticancer
therapies. Combining the high targeting specificity of monoclonal antibodies (mAbs) with …

An improved trypsin digestion method minimizes digestion-induced modifications on proteins

D Ren, GD Pipes, D Liu, LY Shih, AC Nichols… - Analytical …, 2009 - Elsevier
Trypsin digestion can induce artificial modifications such as asparagine deamidation and N-
terminal glutamine cyclization on proteins due to the temperature and the alkaline pH buffers …

Detection, evaluation and minimization of nonenzymatic deamidation in proteomic sample preparation

P Hao, Y Ren, AJ Alpert, SK Sze - Molecular & cellular proteomics, 2011 - ASBMB
Identification of deamidated sites in proteins is commonly used for assignment of N-
glycosylation sites. It is also important for assessing the role of deamidation in vivo …