Harnessing the power of RADseq for ecological and evolutionary genomics

KR Andrews, JM Good, MR Miller, G Luikart… - Nature Reviews …, 2016 - nature.com
High-throughput techniques based on restriction site-associated DNA sequencing (RADseq)
are enabling the low-cost discovery and genoty** of thousands of genetic markers for any …

Opportunities and challenges of macrogenetic studies

DM Leigh, CB van Rees, KL Millette, MF Breed… - Nature Reviews …, 2021 - nature.com
The rapidly emerging field of macrogenetics focuses on analysing publicly accessible
genetic datasets from thousands of species to explore large-scale patterns and predictors of …

ipyrad: Interactive assembly and analysis of RADseq datasets

DAR Eaton, I Overcast - Bioinformatics, 2020 - academic.oup.com
Abstract Summary ipyrad is a free and open source tool for assembling and analyzing
restriction site-associated DNA sequence datasets using de novo and/or reference-based …

Stacks 2: Analytical methods for paired‐end sequencing improve RADseq‐based population genomics

NC Rochette, AG Rivera‐Colón… - Molecular ecology, 2019 - Wiley Online Library
For half a century population genetics studies have put type II restriction endonucleases to
work. Now, coupled with massively‐parallel, short‐read sequencing, the family of RAD …

Pearl millet genome sequence provides a resource to improve agronomic traits in arid environments

RK Varshney, C Shi, M Thudi, C Mariac… - Nature …, 2017 - nature.com
Pearl millet [Cenchrus americanus (L.) Morrone] is a staple food for more than 90 million
farmers in arid and semi-arid regions of sub-Saharan Africa, India and South Asia. We report …

Genomic landscape of the global oak phylogeny

AL Hipp, PS Manos, M Hahn, M Avishai… - New …, 2020 - Wiley Online Library
The tree of life is highly reticulate, with the history of population divergence emerging from
populations of gene phylogenies that reflect histories of introgression, lineage sorting and …

Breaking RAD: An evaluation of the utility of restriction site‐associated DNA sequencing for genome scans of adaptation

DB Lowry, S Hoban, JL Kelley, KE Lotterhos, LK Reed… - 2017 - Wiley Online Library
Understanding how and why populations evolve is of fundamental importance to molecular
ecology. Restriction site‐associated DNA sequencing (RAD seq), a popular reduced …

RAD capture (Rapture): flexible and efficient sequence-based genoty**

OA Ali, SM O'Rourke, SJ Amish, MH Meek, G Luikart… - Genetics, 2016 - academic.oup.com
Massively parallel sequencing has revolutionized many areas of biology, but sequencing
large amounts of DNA in many individuals is cost-prohibitive and unnecessary for many …

These aren't the loci you'e looking for: Principles of effective SNP filtering for molecular ecologists

SJ O'Leary, JB Puritz, SC Willis, CM Hollenbeck… - 2018 - Wiley Online Library
Sequencing reduced‐representation libraries of restriction site‐associated DNA (RAD seq)
to identify single nucleotide polymorphisms (SNP s) is quickly becoming a standard …

Development of high-density genetic maps for barley and wheat using a novel two-enzyme genoty**-by-sequencing approach

JA Poland, PJ Brown, ME Sorrells, JL Jannink - PloS one, 2012 - journals.plos.org
Advancements in next-generation sequencing technology have enabled whole genome re-
sequencing in many species providing unprecedented discovery and characterization of …