Intracellular delivery is a key step in biological research and has enabled decades of
biomedical discoveries. It is also becoming increasingly important in industrial and medical …

In most higher organisms, DNA is modified after synthesis by the enzymatic conversion of
many cytosine residues to 5-methylcytosine. For several years, control of gene activity by …

We have studied the transient expression of a cloned rabbit hemoglobin/31 gene after its
introduction into HeLa cells. Two and one-half days after transfection using the calcium …

Low-stringency hybridization with human glucocorticoid receptor (hGR) complementary
DNA was used to isolate a new gene encoding a predicted 107-kilodalton polypeptide …

The complex of rapamycin with its intracellular receptor, FKBP12, interacts with
RAFT1/FRAP/mTOR, the in vivo rapamycin-sensitive target and a member of the ataxia …

Focal adhesion kinase (FAK) is a widely expressed nonreceptor protein-tyrosine kinase
implicated in integrin-mediated signal transduction pathways and in the process of …

Direct microinjection of DNA by glass micropipettes was used to introduce the Herpes
simplex virus thymidine kinase gene into cultured mammalian cells. When DNA was …

The availability of monoclonal antibodies has revived interest in immunotherapy. The ability
to influence an individual's immune state by administering immunoglobulin of the …

Blot hybridization analysis indicated that NIH 3T3 mouse bladder transformed by high
molecular weight DNAs of a human bladder and a human lung carcinoma cell line …

Biologists commonly wish to introduce a wide range of substances into living cells. Such
substances include biological stains, proteins (antibodies or enzymes), and genetic material …