Cryopreservation has facilitated advancement of biological research by allowing the storage
of cells over prolonged periods of time. While cryopreservation at extremely low …

Mitochondria, fundamental organelles in cell metabolism, and ATP synthesis are
responsible for generating reactive oxygen species (ROS), calcium homeostasis, and cell …

Sperm cryopreservation is a powerful tool for the livestock breeding program. Several
technical attempts have been made to enhance the efficiency of spermatozoa …

Mitochondria are vital organelles with a multifaceted role in cellular bioenergetics,
biosynthesis, signaling and calcium homeostasis. During oxidative phosphorylation, sperm …

Structural and functional damages to mitochondria of frozen-thawed sperm are a typical
cryoinjury, with mitochondrial permeability transition pore (MPTP) formation being the …

Reactive oxygen species (ROS) have been proposed as one of the main causes of the
impairment of fish spermatozoa integrity and functionality during cryopreservation. The high …

Semen conservation methods have been extensively studied for fish species over the last 60
years. Cryopreservation techniques can be divided into two types: one with slow cooling …

The development of cryopreservation techniques has led to important changes in animal
reproductive biotechnology. However, these techniques are associated with cellular and …

The use of α-tocopherol and ascorbic acid in freezing medium prevents lipoperoxidation and
damage by ROS (reactive oxygen species); however, the effects of these antioxidants in …

The objective of this study was to determine the effect of freezing on the function in Atlantic
salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium+ 1. 3M …