Hydrogenases

W Lubitz, H Ogata, O Rudiger, E Reijerse - Chemical reviews, 2014 - ACS Publications
The reaction takes place at a specialized metal center that dramatically increases the acidity
of H2 and leads to a heterolytic splitting of the molecule which is strongly accelerated by the …

NADPH-generating systems in bacteria and archaea

SK Spaans, RA Weusthuis, J Van Der Oost… - Frontiers in …, 2015 - frontiersin.org
Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is an essential electron
donor in all organisms. It provides the reducing power that drives numerous anabolic …

An overview on progress, advances, and future outlook for biohydrogen production technology

KK Brar, AA Cortez, VOA Pellegrini, K Amulya… - International Journal of …, 2022 - Elsevier
The hydrogen (H 2) economy has been recognized globally from a socio-economic, and
environmental viewpoint. Hydrogen can be produced using technologies such as …

[HTML][HTML] [NiFe] hydrogenases: a common active site for hydrogen metabolism under diverse conditions

HS Shafaat, O Rüdiger, H Ogata, W Lubitz - Biochimica et Biophysica Acta …, 2013 - Elsevier
Hydrogenase proteins catalyze the reversible conversion of molecular hydrogen to protons
and electrons. The most abundant hydrogenases contain a [NiFe] active site; these proteins …

Genetic engineering contribution to develo** cyanobacteria-based hydrogen energy to reduce carbon emissions and establish a hydrogen economy

GK Kamshybayeva, BD Kossalbayev… - International Journal of …, 2024 - Elsevier
Growing concerns over greenhouse gas emissions and energy insecurity caused by the
depletion of conventional fuels have led to a search for sustainable fuel alternatives. As an …

The bidirectional NiFe-hydrogenase in Synechocystis sp. PCC 6803 is reduced by flavodoxin and ferredoxin and is essential under mixotrophic, nitrate-limiting …

K Gutekunst, X Chen, K Schreiber, U Kaspar… - Journal of Biological …, 2014 - jbc.org
Cyanobacteria are able to use solar energy for the production of hydrogen. It is generally
accepted that cyanobacterial NiFe-hydrogenases are reduced by NAD (P) H. This is in …

Reversible glutamate coordination to high-valent nickel protects the active site of a [NiFe] hydrogenase from oxygen

CJ Kulka-Peschke, AC Schulz, C Lorent… - Journal of the …, 2022 - ACS Publications
NAD+-reducing [NiFe] hydrogenases are valuable biocatalysts for H2-based energy
conversion and the regeneration of nucleotide cofactors. While most hydrogenases are …

H2 Conversion in the Presence of O2 as Performed by the Membrane‐Bound [NiFe]‐Hydrogenase of Ralstonia eutropha

O Lenz, M Ludwig, T Schubert, I Bürstel… - …, 2010 - Wiley Online Library
Abstract [NiFe]‐hydrogenases catalyze the oxidation of H2 to protons and electrons. This
reversible reaction is based on a complex interplay of metal cofactors including the Ni–Fe …

Hydrogen production by immobilized Synechocystis sp. PCC 6803

E Touloupakis, G Rontogiannis, AMS Benavides… - international journal of …, 2016 - Elsevier
The ability of the immobilized Synechocystis sp. PCC 6803 cells to produce hydrogen via an
indirect light driven process was investigated. Cells were successfully immobilized in a …

Cyanobacterial hydrogenases and hydrogen metabolism revisited: recent progress and future prospects

N Khanna, P Lindblad - International journal of molecular sciences, 2015 - mdpi.com
Cyanobacteria have garnered interest as potential cell factories for hydrogen production. In
conjunction with photosynthesis, these organisms can utilize inexpensive inorganic …