Current practices for reference gene selection in RT-qPCR of Aspergillus: Outlook and recommendations for the future

M Archer, J Xu - Genes, 2021 - mdpi.com
Aspergillus is a genus of filamentous fungi with vast geographic and ecological distributions.
Species within this genus are clinically, agriculturally and biotechnologically relevant …

Identification of reliable reference genes for qRT-PCR in the ephemeral plant Arabidopsis pumila based on full-length transcriptome data

Y **, F Liu, W Huang, Q Sun, X Huang - Scientific Reports, 2019 - nature.com
Arabidopsis pumila, an annual ephemeral plant, plays important roles in preventing wind
and sand erosion, water and soil conservation, and microhabitat improvement in the North of …

Simultaneous sequencing of coding and noncoding RNA reveals a human transcriptome dominated by a small number of highly expressed noncoding genes

V Boivin, G Deschamps-Francoeur, S Couture… - Rna, 2018 - rnajournal.cshlp.org
Comparing the abundance of one RNA molecule to another is crucial for understanding
cellular functions but most sequencing techniques can target only specific subsets of RNA …

Development of reference genes for horticultural plants

U Ahmed, Q **e, X Shi, B Zheng - Critical Reviews in Plant …, 2022 - Taylor & Francis
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is extensively
applied technique to investigate the transcript abundance of target genes in various …

Stability evaluation of reference genes for real-time quantitative PCR normalization in Spodoptera frugiperda (Lepidoptera: Noctuidae)

B Shu, H Yu, J Dai, Z **e, W Qian, J Lin - Journal of Integrative Agriculture, 2021 - Elsevier
Real-time quantitative PCR (qPCR) is a reliable and widely used technique for analyzing the
expression profiles of target genes in different species, and reference genes with stable …

Assessment of reference genes at six different developmental stages of Schistosoma mansoni for quantitative RT-PCR

GO Silveira, MS Amaral, HS Coelho, LF Maciel… - Scientific reports, 2021 - nature.com
Reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) is the
most used, fast, and reproducible method to confirm large-scale gene expression data. The …

Reference Gene Selection for Normalizing Gene Expression in Ips Sexdentatus (Coleoptera: Curculionidae: Scolytinae) Under Different Experimental Conditions

G Sellamuthu, S Amin, J Bílý, J Synek… - Frontiers in …, 2021 - frontiersin.org
Ips sexdentatus (Coleoptera: Curculionidae: Scolytinae) is one of the most destructive and
economically important forest pests. A better understanding of molecular mechanisms …

Selection, identification and evaluation of optimal reference genes in Chinese sturgeon (Acipenser sinensis) under polypropylene microplastics stress

X Cheng, K **ao, W Jiang, G Peng, P Chen… - Science of The Total …, 2024 - Elsevier
Polypropylene microplastics (PP-MPs) are emerging environmental contaminants that have
the potential to cause adverse effects on aquatic organisms. Reverse transcriptase …

Identifying optimal reference genes for gene expression studies in Eurasian spruce bark beetle, Ips typographus (Coleoptera: Curculionidae: Scolytinae)

G Sellamuthu, J Bílý, MR Joga, J Synek, A Roy - Scientific Reports, 2022 - nature.com
Eurasian spruce bark beetle (Ips typographus [L.]) causes substantial damage to spruce
forests worldwide. Undoubtedly, more aggressive measures are necessary to restrict the …

Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata

R Li, K Cui, Q **e, S **e, X Chen, L Zhuo, A Cao… - Scientific Reports, 2021 - nature.com
The desert pioneer plant Stipagrostis pennata plays an important role in sand fixation, wind
prevention, and desert ecosystem recovery. An absence of reference genes greatly limits …