Nucleic acid amplification technologies (NAATs) have become fundamental tools in
molecular diagnostics, due to their ability to detect small amounts of target molecules. Since …

Nucleic acid amplification has permeated every field in the life sciences since the
introduction of the classic polymerase chain reaction (PCR) method in 1983. Yet, despite its …

Recombinase polymerase amplification (RPA) is a highly sensitive and selective isothermal
amplification technique, operating at 37–42° C, with minimal sample preparation and …

Digital Polymerase Chain Reaction (dPCR) is a novel method for the absolute quantification
of target nucleic acids. Quantification by dPCR hinges on the fact that the random …

Isothermal amplification of nucleic acids is a simple process that rapidly and efficiently
accumulates nucleic acid sequences at constant temperature. Since the early 1990s …

BACKGROUND First introduced in 2006, recombinase polymerase amplification (RPA) has
stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them …

The introduction of nucleic acid amplification techniques has revolutionized the field of
medical diagnostics in the last decade. The advent of PCR catalyzed the increasing …

Isothermal exponential amplification techniques, such as strand‐displacement amplification
(SDA), rolling circle amplification (RCA), loop‐mediated isothermal amplification (LAMP) …

This Perspective focuses on the latest strategies and challenges for the development of
bioanalytical sensors with sub-picomolar detection limits. Achieving sub-picomolar detection …

Portable, low-cost, and quantitative nucleic acid detection is desirable for point-of-care
diagnostics; however, current polymerase chain reaction testing often requires time …