[HTML][HTML] Nanopore sequencing technology, bioinformatics and applications

Y Wang, Y Zhao, A Bollas, Y Wang, KF Au - Nature biotechnology, 2021 - nature.com
Rapid advances in nanopore technologies for sequencing single long DNA and RNA
molecules have led to substantial improvements in accuracy, read length and throughput …

DNA methylation in mammalian development and disease

ZD Smith, S Hetzel, A Meissner - Nature Reviews Genetics, 2024 - nature.com
The DNA methylation field has matured from a phase of discovery and genomic
characterization to one seeking deeper functional understanding of how this modification …

Multiple rereads of single proteins at single–amino acid resolution using nanopores

H Brinkerhoff, ASW Kang, J Liu, A Aksimentiev… - Science, 2021 - science.org
A proteomics tool capable of identifying single proteins would be important for cell biology
research and applications. Here, we demonstrate a nanopore-based single-molecule …

The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community

M Jain, HE Olsen, B Paten, M Akeson - Genome biology, 2016 - Springer
Nanopore DNA strand sequencing has emerged as a competitive, portable technology.
Reads exceeding 150 kilobases have been achieved, as have in-field detection and …

Detecting DNA cytosine methylation using nanopore sequencing

JT Simpson, RE Workman, PC Zuzarte, M David… - Nature …, 2017 - nature.com
In nanopore sequencing devices, electrolytic current signals are sensitive to base
modifications, such as 5-methylcytosine (5-mC). Here we quantified the strength of this effect …

Three decades of nanopore sequencing

D Deamer, M Akeson, D Branton - Nature biotechnology, 2016 - nature.com
A long-held goal in sequencing has been to use a voltage-biased nanoscale pore in a
membrane to measure the passage of a linear, single-stranded (ss) DNA or RNA molecule …

DNA methylation-calling tools for Oxford Nanopore sequencing: a survey and human epigenome-wide evaluation

Y Liu, W Rosikiewicz, Z Pan, N Jillette, P Wang… - Genome biology, 2021 - Springer
Background Nanopore long-read sequencing technology greatly expands the capacity of
long-range, single-molecule DNA-modification detection. A growing number of analytical …

Detection of DNA base modifications by deep recurrent neural network on Oxford Nanopore sequencing data

Q Liu, L Fang, G Yu, D Wang, CL **ao… - Nature communications, 2019 - nature.com
DNA base modifications, such as C5-methylcytosine (5mC) and N6-methyldeoxyadenosine
(6mA), are important types of epigenetic regulations. Short-read bisulfite sequencing and …

Review of clinical next-generation sequencing

S Yohe, B Thyagarajan - Archives of pathology & …, 2017 - meridian.allenpress.com
Context.—Next-generation sequencing (NGS) is a technology being used by many
laboratories to test for inherited disorders and tumor mutations. This technology is new for …

[HTML][HTML] Benchmarking of Nanopore R10. 4 and R9. 4.1 flow cells in single-cell whole-genome amplification and whole-genome shotgun sequencing

Y Ni, X Liu, ZM Simeneh, M Yang, R Li - Computational and Structural …, 2023 - Elsevier
Third-generation sequencing can be used in human cancer genomics and epigenomic
research. Oxford Nanopore Technologies (ONT) recently released R10. 4 flow cell, which …