PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions
DNA analysis has seen an incredible development in terms of instrumentation, assays and
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …
DNA polymerases as useful reagents for biotechnology–the history of developmental research in the field
S Ishino, Y Ishino - Frontiers in microbiology, 2014 - frontiersin.org
DNA polymerase is a ubiquitous enzyme that synthesizes complementary DNA strands
according to the template DNA in living cells. Multiple enzymes have been identified from …
according to the template DNA in living cells. Multiple enzymes have been identified from …
A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro
Y Wang, DE Prosen, L Mei, JC Sullivan… - Nucleic acids …, 2004 - academic.oup.com
Mechanisms that allow replicative DNA polymerases to attain high processivity are often
specific to a given polymerase and cannot be generalized to others. Here we report a …
specific to a given polymerase and cannot be generalized to others. Here we report a …
Biotechnology of extremely thermophilic archaea
Although the extremely thermophilic archaea (Topt≥ 70° C) may be the most primitive
extant forms of life, they have been studied to a limited extent relative to mesophilic …
extant forms of life, they have been studied to a limited extent relative to mesophilic …
Engineering polymerases for applications in synthetic biology
A Nikoomanzar, N Chim, EJ Yik… - Quarterly reviews of …, 2020 - cambridge.org
DNA polymerases play a central role in biology by transferring genetic information from one
generation to the next during cell division. Harnessing the power of these enzymes in the …
generation to the next during cell division. Harnessing the power of these enzymes in the …
An Oomycete CRN Effector Reprograms Expression of Plant HSP Genes by Targeting their Promoters
T Song, Z Ma, D Shen, Q Li, W Li, L Su, T Ye… - PLoS …, 2015 - journals.plos.org
Oomycete pathogens produce a large number of CRN effectors to manipulate plant immune
responses and promote infection. However, their functional mechanisms are largely …
responses and promote infection. However, their functional mechanisms are largely …
Recent developments in the optimization of thermostable DNA polymerases for efficient applications
AR Pavlov, NV Pavlova, SA Kozyavkin… - Trends in …, 2004 - cell.com
Thermostable DNA polymerases have a crucial role in current methods of DNA amplification
and sequencing. Major improvements in these methods have been made in recent years …
and sequencing. Major improvements in these methods have been made in recent years …
Improving sequencing quality from PCR products containing long mononucleotide repeats
Stutter products are a common artifact in the PCR amplification of frequently used genetic
markers that contain mononucleotide simple sequence repeats. Despite the importance of …
markers that contain mononucleotide simple sequence repeats. Despite the importance of …
Shark cartilage, cancer and the growing threat of pseudoscience
GK Ostrander, KC Cheng, JC Wolf, MJ Wolfe - Cancer research, 2004 - AACR
The promotion of crude shark cartilage extracts as a cure for cancer has contributed to at
least two significant negative outcomes: a dramatic decline in shark populations and a …
least two significant negative outcomes: a dramatic decline in shark populations and a …
A Novel Branching Enzyme of the GH-57 Family in the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1
T Murakami, T Kanai, H Takata, T Kuriki… - Journal of …, 2006 - Am Soc Microbiol
Branching enzyme (BE) catalyzes formation of the branch points in glycogen and
amylopectin by cleavage of the α-1, 4 linkage and its subsequent transfer to the α-1, 6 …
amylopectin by cleavage of the α-1, 4 linkage and its subsequent transfer to the α-1, 6 …