Structural analysis of resistance-nodulation cell division transporters

PA Klenotic, EW Yu - Microbiology and Molecular Biology Reviews, 2024‏ - journals.asm.org
Infectious bacteria have both intrinsic and acquired mechanisms to combat harmful biocides
that enter the cell. Through adaptive pressures, many of these pathogens have become …

Drug resistance and physiological roles of RND multidrug efflux pumps in Salmonella enterica, Escherichia coli and Pseudomonas aeruginosa

S Yamasaki, M Zwama, T Yoneda… - …, 2023‏ - microbiologyresearch.org
Drug efflux pumps transport antimicrobial agents out of bacteria, thereby reducing the
intracellular antimicrobial concentration, which is associated with intrinsic and acquired …

Cryo-EM structures of the Klebsiella pneumoniae AcrB multidrug efflux pump

Z Zhang, CE Morgan, RA Bonomo, EW Yu - MBio, 2023‏ - journals.asm.org
The continued challenges of the COVID-19 pandemic combined with the growing problem of
antimicrobial-resistant bacterial infections has severely impacted global health. Specifically …

Molecular insights into the determinants of substrate specificity and efflux inhibition of the RND efflux pumps AcrB and AdeB

J Wilhelm, KM Pos - Microbiology, 2024‏ - microbiologyresearch.org
Gram-negative bacterial members of the Resistance Nodulation and cell Division (RND)
superfamily form tripartite efflux pump systems that span the cell envelope. One of the …

Cryo-EM structures of CusA reveal a mechanism of metal-ion export

MA Moseng, M Lyu, T Pipatpolkai, P Glaza… - MBio, 2021‏ - journals.asm.org
Gram-negative bacteria utilize the resistance-nodulation-cell division (RND) superfamily of
efflux pumps to expel a variety of toxic compounds from the cell. The Escherichia coli CusA …

[HTML][HTML] Recent Advances in Antimicrobial Resistance: Insights from Escherichia coli as a Model Organism

Z Zhang, M Wei, B Jia, Y Yuan - Microorganisms, 2024‏ - mdpi.com
Antimicrobial resistance (AMR) represents a critical global health threat, and a thorough
understanding of resistance mechanisms in Escherichia coli is needed to guide effective …

Insights into substrate recognition and export tunnel preferences in the efflux transporter AcrB

F Makhamadjamonov, ME Karolak, L Smyth… - Protein …, 2025‏ - Wiley Online Library
Abstract In Escherichia coli AcrB is a major multidrug exporter, which confers the bacterium
resistance to many antibiotics with diverse structural and chemical proprieties. Studies have …

The mycobacterium lipid transporter MmpL3 is dimeric in detergent solution, SMALPs and reconstituted nanodiscs

S Cioccolo, JD Barritt, N Pollock, Z Hall… - RSC Chemical …, 2024‏ - pubs.rsc.org
The mycobacterial membrane protein large 3 (MmpL3) transports key precursor lipids to the
outer membrane of Mycobacterium species. Multiple structures of MmpL3 from both M …

Electrochemical Detection of Tobramycin Resistance in Escherichia Coli

LC Lopes, A Jiang, M Zarychta, K Wiebe… - Journal of The …, 2024‏ - iopscience.iop.org
The development of techniques to detect the presence of resistance in pathogens are
urgently needed to face the deadly spread of multi-drug-resistant bacteria. The present work …

[HTML][HTML] Bacteriophage LHE83 targeting OmpA as a receptor exhibited synergism with spectinomycin against Escherichia coli

J Zhen, R Liu, C Man, S Xu, W Zhang, L Zou, W Liu… - Poultry Science, 2024‏ - Elsevier
Understanding the characteristics of bacteriophages is crucial for the optimization of phage
therapy. In this study, the biological and genomic characteristics of coliphage LHE83 were …