Activity-based protein profiling (ABPP) is a technique that uses highly selective active-site
targeted chemical probes to label and monitor the state of proteins. ABPP integrates the …

Plants contain numerous glycoconjugates that are metabolized by specific
glucosyltransferases and hydrolyzed by specific glycosidases, some also catalyzing …

Glycosidases are ubiquitous enzymes that catalyze the hydrolysis of glycosidic linkages in
oligosaccharides and glycoconjugates. These enzymes play a vital role in a wide variety of …

Highlights•Electrophiles can show distinct amino-acid selectivities in proteomes.•The
judicious selection of an electrophile is critical to various chemical-proteomic applications.•A …

INTRODUCTION Immunogenic flagellin fragments are a signature of bacterial invasion in
both plants and animals. Plants recognize flagellin fragments via flagellin sensitive 2 (FLS2) …

Probes that covalently label protein targets facilitate the identification of ligand-binding sites.
Lysine residues are prevalent in the proteome, making them attractive substrates for …

Abstract The Nobel Prize in Chemistry for 2022 was awarded to the pioneers of Lego-like
'click chemistry': combinatorial chemistry with remarkable modularity and diversity. It has …

Activity-based protein profiling (ABPP) is an effective technology for the identification and
functional annotation of enzymes in complex biological samples. ABP designs are normally …

Humans express at least two distinct β-glucuronidase enzymes that are involved in disease:
exo-acting β-glucuronidase (GUSB), whose deficiency gives rise to mucopolysaccharidosis …

Activity-based protein profiling (ABPP) has emerged as a powerful strategy to study the
activity of enzymes in complex proteomes. The aim of ABPP is to selectively visualize only …