Integrative studies of 3D genome organization and chromatin structure

M Di Stefano, G Cavalli - Current Opinion in Structural Biology, 2022 - Elsevier
The structural organization of the genome is emerging as a crucial regulator of the cell state,
affecting gene transcription, DNA replication, and repair. Over the last twenty years …

Loop-extrusion and polymer phase-separation can co-exist at the single-molecule level to shape chromatin folding

M Conte, E Irani, AM Chiariello, A Abraham… - Nature …, 2022 - nature.com
Loop-extrusion and phase-separation have been proposed as mechanisms that shape
chromosome spatial organization. It is unclear, however, how they perform relative to each …

Computational approaches from polymer physics to investigate chromatin folding

S Bianco, AM Chiariello, M Conte, A Esposito… - Current opinion in cell …, 2020 - Elsevier
Microscopy and sequencing-based technologies are providing increasing insights into
chromatin architecture. Nevertheless, a full comprehension of chromosome folding and its …

Comparison of the Hi-C, GAM and SPRITE methods using polymer models of chromatin

L Fiorillo, F Musella, M Conte, R Kempfer… - Nature …, 2021 - nature.com
Hi-C, split-pool recognition of interactions by tag extension (SPRITE) and genome
architecture map** (GAM) are powerful technologies utilized to probe chromatin …

Extracting multi-way chromatin contacts from Hi-C data

L Liu, B Zhang, C Hyeon - PLOS Computational Biology, 2021 - journals.plos.org
There is a growing realization that multi-way chromatin contacts formed in chromosome
structures are fundamental units of gene regulation. However, due to the paucity and …

Polymer physics reveals a combinatorial code linking 3D chromatin architecture to 1D chromatin states

A Esposito, S Bianco, AM Chiariello, A Abraham… - Cell Reports, 2022 - cell.com
The mammalian genome has a complex, functional 3D organization. However, it remains
largely unknown how DNA contacts are orchestrated by chromatin organizers. Here, we …

A modern challenge of polymer physics: novel ways to study, interpret, and reconstruct chromatin structure

L Fiorillo, S Bianco, A Esposito, M Conte… - Wiley …, 2020 - Wiley Online Library
The constant development of sophisticated technologies is allowing to dissect three‐
dimensional chromatin structure at high resolution level. The tremendous amount of …

3D genomics and its applications in precision medicine

M Chen, X Liu, Q Liu, D Shi, H Li - Cellular & Molecular Biology Letters, 2023 - Springer
Abstract Three-dimensional (3D) genomics is an emerging discipline that studies the three-
dimensional structure of chromatin and the three-dimensional and functions of genomes. It …

Polymer models are a versatile tool to study chromatin 3D organization

A Esposito, S Bianco, L Fiorillo, M Conte… - Biochemical Society …, 2021 - portlandpress.com
The development of new experimental technologies is opening the way to a deeper
investigation of the three-dimensional organization of chromosomes inside the cell nucleus …

The physics of DNA folding: polymer models and phase-separation

A Esposito, A Abraham, M Conte, F Vercellone… - Polymers, 2022 - mdpi.com
Within cell nuclei, several biophysical processes occur in order to allow the correct activities
of the genome such as transcription and gene regulation. To quantitatively investigate such …